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Generation of photoactivatable fluorescent protein from photoconvertible ancestor

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Abstract

The DendFP protein from Dendronephthya sp. converts from the green to red fluorescent state under UV light. We have obtained the mutant variant of the protein, which, in contrast to original DendFP, tends to be phototransformed from the nonfluorescent form to the green fluorescent state.

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Abbreviations

DendFP:

fluorescent protein from Dendronephthya sp.

EosFP:

fluorescent protein from Lobophyllia hemprichii

GFP:

green fluorescent protein from Aequorea victoria

Kaede:

fluorescent protein from Trachyphyllia geoffroyi

KikGR:

mutant green fluorescent protein from coral Favia favus

mMaple:

mutant fluorescent protein from Clavularia sp.

PAFP:

photoactivatable fluorescent proteins

FP:

fluorescent proteins

PSFP:

phototransformed fluorescent proteins

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Authors and Affiliations

  1. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia

    A. A. Pakhomov, R. V. Chertkova, I. E. Deyev, A. G. Petrenko & V. I. Martynov

Authors
  1. A. A. Pakhomov
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  2. R. V. Chertkova
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  3. I. E. Deyev
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  4. A. G. Petrenko
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  5. V. I. Martynov
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Corresponding author

Correspondence to A. A. Pakhomov.

Additional information

Original Russian Text © A.A. Pakhomov, R.V. Chertkova, I.E. Deyev, A.G. Petrenko, V.I. Martynov, 2017, published in Bioorganicheskaya Khimiya, 2017, Vol. 43, No. 3, pp. 326–329.

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Pakhomov, A.A., Chertkova, R.V., Deyev, I.E. et al. Generation of photoactivatable fluorescent protein from photoconvertible ancestor. Russ J Bioorg Chem 43, 340–343 (2017). https://doi.org/10.1134/S106816201703013X

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  • DOI: https://doi.org/10.1134/S106816201703013X

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