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Modification of recombinant proteins by covalent polysialation illustrated with the example of human insulin

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Abstract

Methods of selective and nonselective covalent immobilization of genetically engineered proteins on molecules of natural polysialic acid are described by the example of human insulin. Such modification increases insulin lifetime in vivo.

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Abbreviations

APSA:

aldehyde of polysialic acid

RP-HPLC:

reverse-phase high-performance liquid chromatography

PSA:

polysialic acid

References

  1. Byrne, B., Donohoe, G.G., and O’Kennedy, R., Drug Discovery Today, 2007, vol. 12, pp. 319–326.

    Article  PubMed  CAS  Google Scholar 

  2. Gregoriadis, G., McCormack, B., Wang, Z., and Lifely, R., FEBS Lett., 1993, vol. 315, pp. 271–276.

    Article  PubMed  CAS  Google Scholar 

  3. Jain, S., Hreczuk-Hirsta, D.H., McCormack, B., Mital, M., Epenetos, A., Laing, P., and Gregoriadis, G., Biochim. Biophys. Acta, 2003, vol. 1622, pp. 42–49.

    PubMed  CAS  Google Scholar 

  4. Bezuglov, V.V., Gretskaya, N.M., Esipov, S.E., Polyakov, N.B., Nikitina, L.A., Buznikov, G.A., and Lauder, J., Rus. J. Bioorg. Chem., 2004, vol. 30, pp. 459–465; Bioorg. Khim., 2004, vol. 30, pp. 512–519.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, V-437, GSP Moscow, 117997, Russia

    V. V. Bezuglov, N. M. Greskaya, M. Yu. Bobrov, M. G. Akimov, E. V. Fomina-Ageeva, G. N. Zinchenko, D. I. Bairamashvili & A. I. Miroshnikov

Authors
  1. V. V. Bezuglov
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  2. N. M. Greskaya
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  3. M. Yu. Bobrov
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  4. M. G. Akimov
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  5. E. V. Fomina-Ageeva
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  6. G. N. Zinchenko
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  7. D. I. Bairamashvili
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  8. A. I. Miroshnikov
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Corresponding author

Correspondence to V. V. Bezuglov.

Additional information

Original Russian Text © V.V. Bezuglov, N.M. Greskaya, M.Yu. Bobrov, M.G. Akimov, E.V. Fomina-Ageeva, G.N. Zinchenko, D.I. Bairamashvili, A.I. Miroshnikov, 2009, published in Bioorganicheskaya Khimiya, 2009, Vol. 35, No. 2, pp. 274–278.

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Bezuglov, V.V., Greskaya, N.M., Bobrov, M.Y. et al. Modification of recombinant proteins by covalent polysialation illustrated with the example of human insulin. Russ J Bioorg Chem 35, 254–257 (2009). https://doi.org/10.1134/S1068162009020150

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  • DOI: https://doi.org/10.1134/S1068162009020150

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