Abstract
We found a new protein haponin (an HLDF-like protein) in promyelocyte HL-60 cells that is immunoreactive to polyclonal antibodies against HLDFβ. Determination of the partial primary structure of the protein allowed us to reveal an immunogenic peptide of haponin and, on the basis of the amino acid sequence of this peptide, the degenerate primers were synthesized, which enabled us to clone the full-size cDNA of haponin. The stable heterologous expression of this cDNA in E. coli cells (Rosetta™ strain) was obtained. Preparations of natural and recombinant proteins exhibited antigenic cross-reactivity to polyclonal antibodies against this peptide.
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Abbreviations
- eIF1a:
-
eukaryotic translation initiation factor 1a
- haponin:
-
HLDF-like protein
- HLDF and HLDFβ:
-
differentiation factors of HL-60 cells
- PCR:
-
polymerase chain reaction
- STAT1:
-
signal transducer and transcription activator 1
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Original Russian Text © A.V. Vonarshenko, V.V. Radchenko, M.V. Gapon, I.L. Rodionov, I.I. Babichenko, D.L. Kakuev, I.D. Artamonov, A.V. Garkovenko, L.G. Dyachkova, V.M. Lipkin, I.A. Kostanyan, 2007, published in Bioorganicheskaya Khimiya, 2007, Vol. 33, No. 6, pp. 653–656.
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Vonarshenko, A.V., Radchenko, V.V., Gapon, M.V. et al. Identification and expression of haponin, a new protein from HL-60 cells. Russ J Bioorg Chem 33, 607–609 (2007). https://doi.org/10.1134/S106816200706012X
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DOI: https://doi.org/10.1134/S106816200706012X