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Crystallization and preliminary X-ray diffraction study of recombinant ribokinase from Thermus Species 2.9

  • Structure of Macromolecular Compounds
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Abstract

Ribokinase from a thermophilic strain of Thermus species 2.9 belonging to the carbohydrate ribokinase family (EC 2.7.1.15) was isolated, purified, and crystallized. The crystallization conditions were found by the vapor-diffusion technique and were then optimized to apply the capillary counter-diffusion technique. The X-ray diffraction data set was collected from the crystals, which were grown by the counter-diffusion technique, at the SPring-8 synchrotron radiation facility to 2.87 Å resolution. The crystals belong to sp. gr. P1211 and have the following unit-cell parameters: a = 81.613 Å, b = 156.132 Å, c = 87.714 Å, α = γ = 90°, β = 103.819°. The X-ray diffraction data set is suitable for determining the three-dimensional structure of the protein by the molecular-replacement method.

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Authors and Affiliations

  1. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia

    Yu. A. Abramchik, T. I. Muravieva & R. S. Esipov

  2. Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics,”, Russian Academy of Sciences, Moscow, 119333, Russia

    V. I. Timofeev & I. P. Kuranova

  3. National Research Centre “Kurchatov Institute,”, Moscow, 123098, Russia

    V. I. Timofeev & I. P. Kuranova

Authors
  1. Yu. A. Abramchik
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  2. V. I. Timofeev
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  3. T. I. Muravieva
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  4. R. S. Esipov
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  5. I. P. Kuranova
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Corresponding author

Correspondence to V. I. Timofeev.

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Original Russian Text © Yu.A. Abramchik, V.I. Timofeev, T.I. Muravieva, R.S. Esipov, I.P. Kuranova, 2016, published in Kristallografiya, 2016, Vol. 61, No. 6, pp. 940–944.

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Abramchik, Y.A., Timofeev, V.I., Muravieva, T.I. et al. Crystallization and preliminary X-ray diffraction study of recombinant ribokinase from Thermus Species 2.9. Crystallogr. Rep. 61, 974–978 (2016). https://doi.org/10.1134/S106377451606002X

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  • DOI: https://doi.org/10.1134/S106377451606002X

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