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Purification, crystallization, and preliminary X-ray diffraction study of purine nucleoside phosphorylase from E. coli

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Abstract

Crystals of E. coli purine nucleoside phosphorylase were grown in microgravity by the capillary counter-diffusion method through a gel layer. The X-ray diffraction data set suitable for the determination of the three-dimensional structure at atomic resolution was collected from one crystal at the Spring-8 synchrotron facility to 0.99 Å resolution. The crystals belong to sp. gr. P21 and have the following unit-cell parameters: a = 74.1 Å, b = 110.2 Å, c = 88.2 Å, α = γ = 90°, β = 111.08°. The crystal contains six subunits of the enzyme comprising a hexamer per asymmetric unit. The hexamer is the biological active form of E. coli. purine nucleoside phosphorylase.

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Authors and Affiliations

  1. Shubnikov Institute of Crystallography, Russian Academy of Sciences, Leninskii pr. 59, Moscow, 119333, Russia

    Yu. A. Abramchik, V. I. Timofeev, N. E. Zhukhlistova & I. P. Kuranova

  2. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

    Yu. A. Abramchik, T. I. Muravieva & R. S. Esipov

  3. National Research Centre “Kurchatov Institute,”, pl. Akademika Kurchatova 1, Moscow, 123098, Russia

    V. I. Timofeev & I. P. Kuranova

Authors
  1. Yu. A. Abramchik
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  2. V. I. Timofeev
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  3. N. E. Zhukhlistova
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  4. T. I. Muravieva
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  5. R. S. Esipov
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  6. I. P. Kuranova
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Corresponding authors

Correspondence to Yu. A. Abramchik, V. I. Timofeev or N. E. Zhukhlistova.

Additional information

Original Russian Text © Yu.A. Abramchik, V.I. Timofeev, N.E. Zhukhlistova, T.I. Muravieva, R.S. Esipov, I.P. Kuranova, 2015, published in Kristallografiya, 2015, Vol. 60, No. 4, pp. 575–578.

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Abramchik, Y.A., Timofeev, V.I., Zhukhlistova, N.E. et al. Purification, crystallization, and preliminary X-ray diffraction study of purine nucleoside phosphorylase from E. coli . Crystallogr. Rep. 60, 521–524 (2015). https://doi.org/10.1134/S1063774515040021

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  • DOI: https://doi.org/10.1134/S1063774515040021

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