Abstract
We proposed a procedure for the IR spectrometric determination of lipid hydroperoxides in biological systems. The main bands in the IR absorption spectra of linoleic acid and its hydroperoxide were identified, and analytical bands suitable for the determination of both compounds in their mixtures were selected. It was demonstrated that tert-butyl hydroperoxide can be used as an external standard for determining fatty acid hydroperoxides. Using the external standard method (calibration curve) for tert-butyl hydroperoxides, we calculated the concentration of linoleic acid hydroperoxide in its mixture with linoleic acid; it agreed with the specified values. Using the developed procedure, we estimated the concentration of hydroperoxide groups in natural cardiolipin. The results were compared to those obtained by an independent method (activated chemiluminescence).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Vladimirov, Yu.A. and Archakov, A.I., Perekisnoe okislenie lipidov v biologicheskikh membranakh (Lipid Peroxidation in Biological Membranes), Moscow Nauka, 1972.
Kagan, V.E., Bayir, H.A., Belikova, N.A., Kapralov, O., Tyurina, Y.Y., Tyurin, V.A., Jiang, J., Stoyanovsky, D.A., Wipf, P., and Kochanek, P.M., Free Radicals Biol. Med., 2009, vol. 46, no. 11, p. 1439.
Kagan, V.E., Tyurin, V.A., Jiang, J., Tyurina, Y.Y., Ritov, V.B., Amoscato, A.A., Osipov, A.N., Belikova, N.A., Kapralov, A.A., and Kini, V.V., Nat. Chem. Biol., 2005, vol. 1, p. 223.
Tyurina, Y.Y., Poloyac, S.M., Tyurin, V.A., Kapralov, A.A., Jiang, J., Anthonymuthu, T.S., Kapralova, V.I., Vikulina, A.S., Jung, M.Y., and Epperly, M.W., Nat. Chem., 2014, vol. 6, no. 6, p. 542.
GOST (State Standard) R 51487-99: Vegetable Oils and Animal Fats. Method for Determination of Peroxide Value, Moscow: Standartinform, 2008, p. 6.
Alpat, S.D., Alpat, S.K., Dursun, Z.F., and Telefoncu, A., J. Appl. Chem., 2008, vol. 39, no. 7, p. 971.
Lagrange, J. and Lagrange, P.F., J. Anal. Methods Chem., 1991, no. 339, p. 452.
Mulchandani, A., Wang, C., and Weetall, H., Anal. Chem., 1995, vol. 67, p. 94.
Sparvero, L.J., Amoscato, A.A., Kochanek, P.M., Pitt, B.R., Kagan, V.E., and Bayir, H., J. Neurochem., 2010, vol. 115, no. 6, p. 1322.
Yamamoto, Y., Kambayashi, Y., and Takako, U., in Free Radical and Antioxidant Protocols, vol. 108 of Methods in Molecular Biology, Armstrong, D., Ed., Humana, 1988, p. 63.
Holley, A.E. and Slater, T.F., Free Radical Res. Commun., 1991, vol. 15, no. 1, p. 51.
Miyazawa, T., Fujimoto, K., and Oikawa, S., Biomed. Chromatogr., 1990, vol. 4, no. 3, p. 131.
Yoshida, S., Zhang, Q.Z., Sakuyama, S., and Matsushima, S., Lipids Health Dis., vol. 8, no. is. 2009, p. 28.
Van de Voort, F.R., Ghetler, A., García-González, D.L., and Li Yue, D., Food Anal. Methods, 2008, vol. 1, p. 153.
Van de Voort, F.R., Ismail, A.A., Sedman, J., Dubois, J., and Nicodemo, T., J. Am. Oil Chem. Soc., 1998, vol. 71, no. 9, p. 921.
Russin, T.A., Boye, J.I., Pham, H.M., and Arcand, Y., J. Food Sci., 2006, vol. 71, no. 7, p. 395.
Nenadis, N., Tsikouras, I., and Xenikakis, P., J. Lipid Sci. Technol., 2013, vol. 115, no. 5, p. 526.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © A.A. Dzhatdoeva, A.M. Polimova, E.V. Proskurnina, M.A. Proskurnin, Yu.A. Vladimirov, 2016, published in Zhurnal Analiticheskoi Khimii, 2016, Vol. 71, No. 6, pp. 570–576.
Rights and permissions
About this article
Cite this article
Dzhatdoeva, A.A., Polimova, A.M., Proskurnina, E.V. et al. Determination of lipids and their oxidation products by IR spectrometry. J Anal Chem 71, 542–548 (2016). https://doi.org/10.1134/S1061934816060058
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1061934816060058