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Sensitive spectrofluorimetric determination of alfuzosin in pharmaceutical preparations and human urine using dansyl chloride

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Abstract

A sensitive and selective spectrofluorimetric method was developed for the determination of alfuzosin in pharmaceutical preparations and urine. The method is based on reaction of alfuzosin with 1-dimethylaminonaphthalene-5-sulphonyl (dansyl) chloride in carbonate buffer of pH 9.7 to yield a highly fluorescent derivative that is measured at 516 nm after excitation at 405 nm. The developed method was validated according to the criteria for analytical method, showing good performances in terms of linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, recovery and robustness. The fluorescence intensity versus concentration plot was rectilinear over the range of 25 to 500 ng/mL. The LOD and LOQ were found to be 8 and 25 ng/mL, respectively. The method was successfully applied to the determination of alfuzosin in pharmaceutical preparations and urine.

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References

  1. Miner, M., Rosenberg, M.T., and Perelman, M.A., Clin. Ther., 2004, vol. 28, p. 13.

    Article  Google Scholar 

  2. The Merck Index. 13th ed. Merck and Co Inc., New York: White House Station, 2001. p. 45.

  3. Krishna, M.V. and Sankar, D.G., E-J. Chem., 2007, vol. 4, p. 397.

    Article  CAS  Google Scholar 

  4. Guinebault, P., Broquaire, M., Colafranceschi, C., and Thénot, J.P., J. Chromatogr., 1986, vol. 353, p. 361.

    Article  CAS  Google Scholar 

  5. Krstulovic, A.M. and Vende, J.L., Chirality, 1989, vol. 1, p. 243.

    Article  CAS  Google Scholar 

  6. Rouchouse, A., Manoha, M., Durand, A., and Thénot, J.P., J. Chromatogr., 1989, vol. 506, p. 601.

    Article  Google Scholar 

  7. Desager, J.P., Harvengt, C., Bianchetti, G., and Rosenzweig, P., Int. J. Clin. Pharmacol. Ther. Toxicol., 1993, vol. 31, p. 568.

    CAS  Google Scholar 

  8. Carlucci, G., Di Giuseppe, E., and Mazzeo, P., J. Liq. Chromatogr., 1994, vol. 17, p. 3989.

    Article  CAS  Google Scholar 

  9. Mistri, H.N., Jangid, A.G., Pudageb, A., Rathodb, D.M., and Shrivastava, P.S., J. Chromatogr., 2008, vol. 876, p. 236.

    CAS  Google Scholar 

  10. Walker, J.M., Methods Mol. Biol., 1994, vol. 32, p. 321.

    CAS  Google Scholar 

  11. Walker, J.M., Methods Mol. Biol., 1994, vol. 32, p. 329.

    CAS  Google Scholar 

  12. Bartzatt, R., J. Biochem. Biophys. Methods., 2001, vol. 47, p. 189.

    Article  CAS  Google Scholar 

  13. Validation of Analytical Procedures. Methodology, Food and Drug Administration / Ed. Rockville, M.D., Guidance for Industry: ICH-Q2B, 1997.L

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science and Letters, Namik Kemal University, Tekirdag, Turkey

    Ayça Karasakal

  2. Department of Analytical Chemistry, Faculty of Pharmacy, Istanbul University, 34416, Istanbul, Turkey

    Sevgi Tatar Ulu

Authors
  1. Ayça Karasakal
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  2. Sevgi Tatar Ulu
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Correspondence to Sevgi Tatar Ulu.

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Karasakal, A., Ulu, S.T. Sensitive spectrofluorimetric determination of alfuzosin in pharmaceutical preparations and human urine using dansyl chloride. J Anal Chem 70, 708–711 (2015). https://doi.org/10.1134/S1061934815060040

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  • DOI: https://doi.org/10.1134/S1061934815060040

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