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Effect of Low Concentrations of Thrombin on the Dynamic Surface Properties of Fibrinogen Solutions

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Abstract

The interaction between fibrinogen and thrombin with the formation of fibrin is a key stage in the formation of a blood clot during blood coagulation, with the morphology of the clot being determined by the concentrations of the components. It has previously been shown that, at low thrombin concentrations, long fibrillar aggregates of fibrin are formed. In this paper, we consider the features of the formation of fibrin aggregates in the surface layers of aqueous solutions at relatively low concentrations of both fibrinogen (5 × 10–9–2 × 10–7 M) and thrombin (5–25 U/L). At a low thrombin concentration (5 U/L), nonmonotonic dependences of elasticity are observed probably due to the unfolding of protein macromolecules in the surface layer. At higher enzyme concentrations (10 and 25 U/L), these dependences become monotonic, and the dynamic surface elasticity reaches higher values that exceed those for pure protein solutions. Atomic force microscopic examinations have suggested that this effect is caused by the formation of fibrillar aggregates in the surface layer.

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ACKNOWLEDGMENTS

We are grateful to Prof. B.A. Noskov for discussing the results of this work. The authors are also grateful to the resource centers of St. Petersburg State University (Center for Optical and Laser Research, Interdisciplinary Resource Center for Nanotechnologies, Center for Methods of Analysis of Substance Composition, Center for Thermogravimetric and Calorimetric Research Methods, and Center for Diagnostics of Functional Materials for Medicine, Pharmacology and Nanoelectronics) for the possibility to use their equipment.

Funding

This work was supported by the Grant from the President of the Russian Federation for State Support of Young Russian Scientists (project no. MK-2176.2021.1.3).

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Authors and Affiliations

  1. Department of Colloid Chemistry, St. Petersburg State University, 198504, St. Petersburg, Russia

    O. Yu. Milyaeva & A. R. Rafikova

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  1. O. Yu. Milyaeva
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  2. A. R. Rafikova
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Correspondence to O. Yu. Milyaeva.

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Milyaeva, O.Y., Rafikova, A.R. Effect of Low Concentrations of Thrombin on the Dynamic Surface Properties of Fibrinogen Solutions. Colloid J 84, 55–63 (2022). https://doi.org/10.1134/S1061933X22010070

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  • DOI: https://doi.org/10.1134/S1061933X22010070

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