Abstract
The shaggy gene of Drosophila melanogaster encodes highly conserved serine-threonine protein kinase GSK3 (Glycogen Syntase Kinase 3), which plays an important role in different signaling pathways and metabolic processes. This study is the first to demonstrate that shaggy mutations affect lifespan, with an increase in longevity associated with a decrease in synapse activity. The results from this study on changes in shaggy expression in mutants suggest that the observed phenotypic changes are based on an alternation in the expression of minor shaggy transcripts induced by mutational changes in their regulatory region.
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ACKNOWLEDGMENTS
We thank L.V. Olenina for consultations and discussion of the study. We are grateful to the staff of the Bloomington Drosophila Stock Center (Bloomington, USA, https:// bdsc.indiana.edu/index.html) for many years of assistance to our research.
This study was carried out using the equipment of the Shared Research Center of the Institute of Molecular Genetics of the Russian Academy of Sciences.
Funding
This study was supported by the state contract with the Institute of Molecular Genetics of the Russian Academy of Sciences (state registration no. AAAA-A19-119022590053-3) and by the Russian Foundation for Basic Research (grant nos. 18-34-00934-mol_a, 19-34-80042-mol_ev_a).
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Trostnikov, M.V., Veselkina, E.R., Krementsova, A.V. et al. Insertion Mutations of the shaggy Gene, Encoding Protein Kinase GSK3, Extend Drosophila melanogaster Lifespan. Russ J Genet 55, 1165–1170 (2019). https://doi.org/10.1134/S1022795419090163
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DOI: https://doi.org/10.1134/S1022795419090163