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Physiological features of rapeseed plants expressing the gene for an antimicrobial peptide cecropin P1

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Abstract

Transgenic rapeseed (Brassica napus L.) plants carrying an artificial gene for the antimicrobial peptide cecropin P1 (cecP1) were obtained and characterized. The agrobacterial transformation was done by vacuum infiltration of seeds with agrobacterium GV3101(pMP90RK) containing a binary vector pGA482::cecP1. The cec1 gene expression was analyzed by Western blotting and confirmed by antimicrobial activity measurements of plant extracts. The obtained plants showed the resistance to the bacterial and fungal pathogens Erwinia carotovora and Fusarium sporotrichioides. The photosynthetic activities of control and transgenic plants under biotic stress conditions of E. carotovora infection were comparatively studied. The higher tolerance of the cecP1 plants to the oxidative stress caused by paraquat was shown. The results obtained point to the possibility of incorporation of the cecropin P1 gene into the integral stress protection system of plants.

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Abbreviations

cecP1:

cecropin P1

DETC:

diethylthiocarbamate

DF:

delayed fluorescence of chlorophyll a

Km:

kanamycin

NBT:

nitoblue tetrazolium

nptII :

neomycintransferase II

SOD:

superoxide dismutase

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino Branch, Russian Academy of Sciences, pr. Nauki 6, Pushchino, Moscow oblast, 142290, Russia

    N. S. Zakharchenko, Ya. I. Buryanov, A. A. Lebedeva & S. V. Pigoleva

  2. Tula State University, Tula, Russia

    D. V. Vetoshkina & M. A. Chepurnova

  3. Pushchino State Institute of Natural Sciences, Pushchino, Moscow oblast, Russia

    E. V. Loktyushov

  4. Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia

    V. D. Kreslavski & A. A. Kosobryukhov

Authors
  1. N. S. Zakharchenko
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  2. Ya. I. Buryanov
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  3. A. A. Lebedeva
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  4. S. V. Pigoleva
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  5. D. V. Vetoshkina
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  6. E. V. Loktyushov
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  7. M. A. Chepurnova
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  8. V. D. Kreslavski
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  9. A. A. Kosobryukhov
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Correspondence to N. S. Zakharchenko.

Additional information

Original Russian Text © N.S. Zakharchenko, Ya.I. Buryanov, A.A. Lebedeva, S.V. Pigoleva, D.V. Vetoshkina, E.V. Loktyushov, M.A. Chepurnova, V.D. Kreslavski, A.A. Kosobryukhov, 2013, published in Fiziologiya Rastenii, 2013, Vol. 60, No. 3, pp. 424–433.

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Zakharchenko, N.S., Buryanov, Y.I., Lebedeva, A.A. et al. Physiological features of rapeseed plants expressing the gene for an antimicrobial peptide cecropin P1. Russ J Plant Physiol 60, 411–419 (2013). https://doi.org/10.1134/S1021443713030163

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