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Molecular genetic analysis of DNA polymerase and thymidine kinase genes of a HSV-1 population using an MPS technology

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Abstract

Real-time PCR was used to determine the ratio of viral and host DNA in lysates of Vero cells infected with HSV-1 strain L2. The number of virus copies reached a maximum after 24 h of incubation. Total isolated DNA was sequenced using the massively parallel sequencing technique on an Ion Torrent apparatus. Nucleotide sequences of thymidine kinase (UL23) and DNA polymerase (UL30) genes of a HSV-1 L2 population were determined; their primary structures were compared to those of other standard HSV-1 strains, KOS and 17. The detected differences between the UL23 and UL30 sequences of L2 and reference strains KOS and 17 were unimportant because these substitutions did not affect the conserved gene regions.

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Abbreviations

HSV-1:

herpes simplex virus type 1

TK:

thymidine kinase

PCR:

polymerase chain reaction

MPS:

massively parallel sequencing

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Authors and Affiliations

  1. Research Center for Medical Genetics, Russian Academy of Medical Sciences, Moscow, 115478, Russia

    A. A. Gus’kova, M. Yu. Skoblov, A. V. Lavrov & D. V. Goldshtein

  2. Moscow State Medical and Dental University, Moscow, 127473, Russia

    M. Yu. Skoblov

  3. Moscow Institute of Physics and Technology, Dolgoprudnyi, Moscow Region, 141700, Russia

    D. A. Zubtsov

  4. Ivanovskii Institute of Virology, Russian Academy of Medical Sciences, Moscow, 123098, Russia

    V. L. Andronova & G. A. Galegov

  5. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia

    Yu. S. Skoblov

Authors
  1. A. A. Gus’kova
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  2. M. Yu. Skoblov
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  3. A. V. Lavrov
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  4. D. A. Zubtsov
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  5. V. L. Andronova
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  6. D. V. Goldshtein
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  7. G. A. Galegov
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  8. Yu. S. Skoblov
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Corresponding author

Correspondence to A. A. Gus’kova.

Additional information

Original Russian Text © A.A. Gus’kova, M.Yu. Skoblov, A.V. Lavrov, D.A. Zubtsov, V.L. Andronova, D.V. Goldshtein, G.A. Galegov, Yu.S. Skoblov, 2013, published in Molekulyarnaya Biologiya, 2013, Vol. 47, No. 4, pp. 602–608.

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Gus’kova, A.A., Skoblov, M.Y., Lavrov, A.V. et al. Molecular genetic analysis of DNA polymerase and thymidine kinase genes of a HSV-1 population using an MPS technology. Mol Biol 47, 524–529 (2013). https://doi.org/10.1134/S0026893313040067

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  • DOI: https://doi.org/10.1134/S0026893313040067

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