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Formation of Ag/AgCl nanoparticles in the matrix of the exopolysaccharide of a diazotrophic strain Azotobacter chroococcum XU1

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Abstract

A complex of Ag/AgCl nanoparticles was synthesized on the basis of the extracellular polysaccharide of Azotobacter chroococcum XU1 and 10 mM AgNO3 solution. The complex was characterized by UV spectroscopy, X-ray diffraction, and scanning electron microscopy. Colloidal solutions of the complex had absorption peaks at 260 and 420 nm, indicating the formation Ag/AgCl nanoparticles. The size of the nanoparticles varied from 6 to 50 nm. The nanobiocomposite consisting of the exopolysaccharide matrix and Ag/AgCl nanoparticles exhibited a fungicidal effect against such plant pathogens as Fusarium oxysporum f. sp. vasinfectum and Verticillium dahliae.

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References

  • Abbasi, A.R. and Morsali, A., Synthesis and characterization of AgCl nanoparticles under various solvents by ultrasound method, J. Inorg. Organometal. Polym. Mater., 2013, no. 23, pp. 286–292.

    Article  CAS  Google Scholar 

  • Adame-Garcia, J., Luna-Rodriguez, M., and Iglesias-Andreu, L.G., Vanilla rhizobacteria as antagonists against Fusarium oxysporum f. sp. vanillae, Int. J. Agricult. Biol., 2016, vol. 18, pp. 23–30.

    Article  CAS  Google Scholar 

  • An, C., Peng, S., and Sun, Y., Facile synthesis of sunlightdriven AgCl: Ag plasmonic nanophotocatalyst, Adv. Materials, 2010, vol. 22, pp. 2570–2574.

    Article  CAS  Google Scholar 

  • Chetverikov, S.P., Loginov, Ya.O., Pigil’tsova, S.A., Cherkasova, D.V., and Loginov, O.N., Optimization of conditions for cultivation and exopolysaccharide synthesis by Azotobacter vinelandii, Bashkir. Khim. Zh., 2006, no. 13, pp. 8–12.

    CAS  Google Scholar 

  • Dong, Y.-Y., Deng, F., Zhao, J.-J., He, J., Ma, M.-G., Xu, F., and Sun, R.-C., Environmentally friendly ultrasound synthesis and antibacterial activity of cellulose/Ag/AgCl hybrids, Carbohydr. Polymers, 2014, vol. 99, pp. 166–172.

    Article  CAS  Google Scholar 

  • Hu, W., Chen, S., Li, X., Shi, S., Shen, W., Zhang, X., and Wang, H., In situ synthesis of silver chloride nanoparticles into bacterial cellulose membranes, Mater. Sci. Engin. C, 2009, vol. 29, pp. 1216–1219.

    Article  CAS  Google Scholar 

  • Kanmani, P. and Lim, S.T., Synthesis and structural characterization of silver nanoparticles using bacterial exopolysaccharide and its antimicrobial activity against food and multidrug resistant pathogens, Process Biochem., 2013, vol. 48, pp. 1099–1106.

    Article  CAS  Google Scholar 

  • Khan, Z., Al-Thabaiti, S.A., Obaid, A.Y., and Al-Youbi, A.O., Preparation and characterization of silver nanoparticles by chemical reduction method, Colloids Surfaces B: Biointerfaces, 2011, vol. 82, pp. 513–517.

    Article  CAS  PubMed  Google Scholar 

  • Lee, K.Y. and Won, T.P., Green synthesis and antimicrobial activity of silver chloride nanoparticles stabilized with chitosan oligomer, J. Mater. Sci.: Materials in Medicine, 2014, vol. 12, pp. 26–29.

    Google Scholar 

  • Liu, C., Yang, D., Wang, Y., Shi, J., and Jiang, Z., Fabrication of antimicrobial bacterial cellulose–Ag/AgCl nanocomposite using bacteria as versatile biofactory, J. Nanoparticle Res., 2012, vol. 14, p. 1084.

    Article  Google Scholar 

  • Rasulov, B.A., Pattaeva, M.A., Yili, A., and Aisa, H.A., Polysaccharide-based bioflocculant template of a diazotrophic Bradyrhizobum japonicum 36 for controlled assembly of AgCl nanoparticles, Int. J. Biol. Macromol., 2016, vol. 89, pp. 682–688.

    Article  CAS  PubMed  Google Scholar 

  • Rasulov, B.A., Yili, A., and Aisa, H.A., Biosorption of metal ions by exopolysaccharide produced by Azotobacter chroococcum XU1, J. Environ Protection, 2013, vol. 4, pp. 989–993.

    Article  CAS  Google Scholar 

  • Rodriguez-Sanchez, L., Blanco, M.C., and Lopez-Quintela, M.A., Electrochemical synthesis of silver nanoparticles, J. Phys. Chem. B, 2000, vol. 104, pp. 9683–9686.

    Article  CAS  Google Scholar 

  • Zhang, W., Qiao, X., and Chen, J., Synthesis and characterization of silver nanoparticles in AOT microemulsion system, Chem. Phys., 2006, vol. 30, pp. 495–500.

    Article  Google Scholar 

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Authors and Affiliations

  1. Xinjiang Institute of Ecology and Geography, Urumqi, PR China

    B. A. Rasulov & Li Wen Jun

  2. Institute of Genetics and Plants Experimental Biology, Uzbekistan Academy of Sciences, Toshkent, Uzbekistan

    B. A. Rasulov

  3. National University of Uzbekistan named after Mirzo Ulughbek, Tashkent city, Uzbekistan

    K. D. Davranov

Authors
  1. B. A. Rasulov
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  2. K. D. Davranov
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  3. Li Wen Jun
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Correspondence to B. A. Rasulov.

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Original Russian Text © B.A. Rasulov, K.D. Davranov, Li Wen Jun, 2017, published in Mikrobiologiya, 2017, Vol. 86, No. 2, pp. 182–187.

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Rasulov, B.A., Davranov, K.D. & Jun, L.W. Formation of Ag/AgCl nanoparticles in the matrix of the exopolysaccharide of a diazotrophic strain Azotobacter chroococcum XU1. Microbiology 86, 197–201 (2017). https://doi.org/10.1134/S0026261717020175

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  • DOI: https://doi.org/10.1134/S0026261717020175

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