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Specificity of the chitinolytic microbial complex of soils incubated at different temperatures

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Abstract

The structural and functional specificity of the chitinolytic microbial complex changes dramatically depending on the incubation temperature of soil microcosms. It was shown that the highest rates of chitin degradation occurred in desert soils at high temperatures (50°C); in the moderate and northern zones, these rates peaked at lower temperatures (5°C). The role of prokaryotes as the main chitin degraders in soils incubated at high temperatures, with fungi more actively participating in chitin decomposition at low temperatures, was shown for the first time. Fluorescent in situ hybridization (FISH) revealed the predominance of actinomycetes in the metabolically active chitinolytic prokaryotic complex of desert soils (high temperatures); in the soils of the northern latitudes (low temperatures), proteobacteria prevailed. The relationship between the taxonomic position of the dominant members of the chitinolytic complex of soil microorganisms, isolated in pure cultures with the dominant phylogenetic groups and the sequence types obtained by using molecular biological techniques (FISH) was revealed.

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Authors and Affiliations

  1. Department of Soil Biology, Faculty of Soil Science, Moscow State University, Moscow, 119992, Russia

    N. A. Manucharova, A. N. Vlasenko & D. G. Zvyagintsev

  2. Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia

    E. V. Men’ko

Authors
  1. N. A. Manucharova
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  2. A. N. Vlasenko
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  3. E. V. Men’ko
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  4. D. G. Zvyagintsev
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Correspondence to N. A. Manucharova.

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Original Russian Text © N.A. Manucharova, A.N. Vlasenko, E.V. Men’ko, D.G. Zvyagintsev, 2011, published in Mikrobiologiya, 2011, Vol. 80, No. 2, pp. 219–229.

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Manucharova, N.A., Vlasenko, A.N., Men’ko, E.V. et al. Specificity of the chitinolytic microbial complex of soils incubated at different temperatures. Microbiology 80, 205–215 (2011). https://doi.org/10.1134/S002626171102010X

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