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Description of Anaerobacillus alkalilacustre gen. nov., sp. nov.—Strictly anaerobic diazotrophic bacillus isolated from soda lake and transfer of Bacillus arseniciselenatis, Bacillus macyae, and Bacillus alkalidiazotrophicus to Anaerobacillus as the new combinations A. arseniciselenatis comb. nov., A. macyae comb. nov., and A. alkalidiazotrophicus comb. nov.

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Abstract

An anaerobic, spore-forming bacterium (strain Z-0521) was isolated from the iron-reducing microbial community enriched from sample of bottom sediments from low-mineralized soda lake Khadyn, Tuva upper Yenisey region (Russia). Cells of strain Z-0521 are motile straight Gram-positive rods, 0.7–1.1 (µm in diameter and 3.0–7.0 µm length. It is a mesophilic halotolerante obligate alkaliphilic bacterium with a pH range for growth 8.5–10.7 (optimum at 9.6–9.7). Utilizes carbohydrates. Peptides, organic acids or alcohols are not utilized. In the presence of mannite strain Z-0521 reduces AQDS, arsenate, selenate and selenite. It is capable of N2 fixation and has nitrogenase gene nifH. The dominant cellular fatty acids are C16:0, C16:1w7c and Ca15. The G+C content in the DNA is 36.2 mol %. 16S rRNA gene sequencing identified strain Z-0521 as a member of rRNA group 6 of the genus Bacillus. Its closest relatives are B. alkalidiazotrophicus and B. macyae (98.3 and 98.1% sequence similarity). On the basis of physiological properties and genetic analysis, it is proposed that strain Z-0521T should be assigned to a new species of a new genus, Anaerobacillus alkalilacustre gen. nov., sp. nov. It is also proposed that Bacillus arseniciselenatis, Bacillus macyae and Bacillus alkalidiazotrophicus should be transferred to this new genus, with Anaerobacillus arseniciselenatis (formely Bacillus arseniciselenatis) as the type species.

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Octyabrya 7, k. 2, Moscow, 117811, Russia

    D. G. Zavarzina, T. P. Tourova & T. N. Zhilina

  2. Bioengineering Center, Russian Academy of Sciences, pr. 60-letiya Octyabrya 7, k. 1, Moscow, 117811, Russia

    T. V. Kolganova & E. S. Boulygina

Authors
  1. D. G. Zavarzina
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  2. T. P. Tourova
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  3. T. V. Kolganova
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  4. E. S. Boulygina
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  5. T. N. Zhilina
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Correspondence to D. G. Zavarzina.

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Zavarzina, D.G., Tourova, T.P., Kolganova, T.V. et al. Description of Anaerobacillus alkalilacustre gen. nov., sp. nov.—Strictly anaerobic diazotrophic bacillus isolated from soda lake and transfer of Bacillus arseniciselenatis, Bacillus macyae, and Bacillus alkalidiazotrophicus to Anaerobacillus as the new combinations A. arseniciselenatis comb. nov., A. macyae comb. nov., and A. alkalidiazotrophicus comb. nov.. Microbiology 78, 723–731 (2009). https://doi.org/10.1134/S0026261709060095

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