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Rhodobaca barguzinensis sp. nov., a new alkaliphilic purple nonsulfur bacterium isolated from a soda lake of the Barguzin Valley (Buryat Republic, Eastern Siberia)

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Abstract

A novel strain, alga-05, of alkaliphilic purple nonsulfur bacteria was isolated from sediments of a small saline (60 g/l) soda lake near Lake Algin (Barguzin Valley, Buryat Republic, Russia). These bacteria contain bacteriochlorophyll a and carotenoids of the alternative spirilloxanthin group with predominating demethylspheroidenone. They are facultative anaerobes; their photosynthetic structures are of the vesicular type and arranged along the cell periphery. Growth of this strain is possible in a salinity range of 5–80 g/l NaCl, with an optimum at 20 g/l NaCl. Best growth occurred at 20–35°C. Analysis of the 16S rRNA gene sequences demonstrated that the studied isolate is closely related to the alkaliphilic purple nonsulfur bacterium Rhodobaca bogoriensis (99% similarity) isolated from soda lakes of the African Rift Zone. According to the results of DNA-DNA hybridization, strain alga-05 has a 52% similarity with the type species of the genus Rhodobaca. On the basis of the obtained genotypic data and some phenotypic properties (dwelling in a hypersaline soda lake of Siberia, moderate halophily, ability to grow at relatively low temperatures, etc.), the isolated strain of purple bacteria was described as a new species of the genus Rhodobaca, Rca. barguzinensis sp. nov.

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia

    E. N. Boldareva & V. M. Gorlenko

  2. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow oblast, 142290, Russia

    V. N. Akimov

  3. Institute of Fundamental Problems of Biology, Russian Academy of Sciences, ul. Institutskaya 2, Pushchino, Moscow oblast, 142290, Russia

    V. A. Boychenko, A. A. Moskalenko & Z. K. Makhneva

  4. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, Russia

    I. N. Stadnichuk

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  1. E. N. Boldareva
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  2. V. N. Akimov
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  3. V. A. Boychenko
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  4. I. N. Stadnichuk
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  5. A. A. Moskalenko
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  6. Z. K. Makhneva
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  7. V. M. Gorlenko
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Correspondence to E. N. Boldareva.

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Original Russian Text © E.N. Boldareva, V.N. Akimov, V.A. Boychenko, I.N. Stadnichuk, A.A. Moskalenko, Z.K. Makhneva, V.M. Gorlenko, 2008, published in Mikrobiologiya, 2008, Vol. 77, No. 2, pp. 241–254.

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Boldareva, E.N., Akimov, V.N., Boychenko, V.A. et al. Rhodobaca barguzinensis sp. nov., a new alkaliphilic purple nonsulfur bacterium isolated from a soda lake of the Barguzin Valley (Buryat Republic, Eastern Siberia). Microbiology 77, 206–218 (2008). https://doi.org/10.1134/S0026261708020148

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