Abstract
The conditions for sensitive and selective determination of tetracyclines in milk in the form of their complexes with Mg2+ via the HPLC method with post-column derivatization and fluorescence detection are found. It is shown that the fluorescence of tetracycline-Mg2+ complexes in microemulsions is 1.8 times more intense than that in aqueous acetonitrile medium. The detection limits of tetracycline, oxytetracycline, and doxycycline are 5, 8 and 25 ng mL–1, respectively.
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Original Russian Text © I.D. Kargin, L.S. Sokolova, A.V. Pirogov, O.A. Shpigun, 2015, published in Zavodskaya Laboratoriya, Diagnostika Materialov, 2015, Vol. 81, No. 2, pp. 5–9.
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Kargin, I.D., Sokolova, L.S., Pirogov, A.V. et al. HPLC determination of tetracycline antibiotics in milk with post-column derivatization and fluorescence detection. Inorg Mater 52, 1365–1369 (2016). https://doi.org/10.1134/S0020168516140065
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DOI: https://doi.org/10.1134/S0020168516140065