Abstract
The effect of different pressure levels of a gas mixture of carbon monoxide and oxygen on the shelf life of rat heart tissue during prolonged hypothermic (4°C) gas preservation with the durations of sample storage of 24, 36, and 48 h was evaluated. The pressure decrease of the CO/O2 gas mixture (in the ratio of 1 : 1) from 6.5 to 1.5 atm during 24 h preservation led to the appearance of myocardial infarction zones (17.74 ± 3.5% of the total sample area) and a violation of the contractile function of the ventricles. At the same time, the indicators of the heart shelf life after storage under pressure of 3.5 and 6.5 atm did not significantly differ. The advantage of the pressure of 6.5 atm compared to the pressure of 3.5 atm during 36 and 48 h preservation was observed. The myocardial infarction zones after 48 h of preservation were 33.65 ± 11.96% and 61.92 ± 6.38%, respectively. Exploratory introduction of argon to the gas mixture (CO : O2 : Ar = 2 : 2 : 1) for a possible synergistic increase in organoprotection in the experiments on heart preservation under the pressure of 3.5 atm did not result in significant improvement in the index of heart tissue shelf life. The potential mechanisms of the protective effect of the mixture of carbon monoxide and oxygen in hypothermic preservation are discussed.
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The research was carried out with the financial support of the Russian Foundation for Basic Research, project no. 20-34-90132/20 dated August 18, 2020.
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Statement on the welfare of animals. The studies were conducted in accordance with the requirements of the European Convention for the Protection of Animals 2010/63/EU.
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Gurin, A.E., Gagarinsky, E.L. & Fesenko, E.E. The Effect of Pressure of Gas Mixtures Containing Carbon Monoxide, Oxygen and Argon on the Shelf Life of Rat Heart Tissues in Hypothermic Conditions. BIOPHYSICS 66, 821–827 (2021). https://doi.org/10.1134/S0006350921050079
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DOI: https://doi.org/10.1134/S0006350921050079