Skip to main content
SpringerLink
Log in

Identification and mapping of ten new potential insulators in the FXYD5-COX7A1 region of human chromosome 19q13.12

  • Published:
Biochemistry (Moscow) Aims and scope Submit manuscript

Abstract

A positive-negative selection system revealed 10 potential insulators able to block enhancer interaction with promoter in the 106 bp human chromosome 19 region between genes FXYD5 and COX7A1. Relative positions of insulators and genes are in accord with the hypothesis that insulators subdivide genomic DNA into independently regulated loop domains.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

CMV:

cytomegalovirus

GANC:

2-amino-1,9-dihydro-9-((2-hydroxy-1-(hydroxymethyl)ethoxy)methyl)-6H-purine-6-one (ganciclovir)

References

  1. Akopov, S. B., Chernov, I. P., Bulanenkova, S. S., Skvortsova, Yu. V., Vetchinova, A. S., and Nikolaev, L. G. (2007) Biochemistry (Moscow), 72, 589–594.

    Article  CAS  Google Scholar 

  2. Akopov, S. B., Chernov, I. P., Vetchinova, A. S., Bulanenkova, S. S., and Nikolaev, L. G. (2007) Mol. Biol. (Moscow), 41, 717–722.

    Article  CAS  Google Scholar 

  3. Maximenko, O. G., Chetverina, D. A., and Georgiev, P. G. (2006) Russ. J. Genet., 42, 845–857.

    Article  CAS  Google Scholar 

  4. Dorman, E. R., Bushey, A. M., and Corces, V. G. (2007) Semin. Cell. Dev. Biol., 18, 682–690.

    Article  PubMed  CAS  Google Scholar 

  5. Wallace, J. A., and Felsenfeld, G. (2007) Curr. Opin. Genet. Dev., 17, 400–407.

    Article  PubMed  CAS  Google Scholar 

  6. Emery, D. W., Yannaki, E., Tubb, J., Nishino, T., Li, Q., and Stamatoyannopoulos, G. (2002) Blood, 100, 2012–2019.

    Article  PubMed  CAS  Google Scholar 

  7. Nagaya, S., Yoshida, K., Kato, K., Akasaka, K., and Shinmyo, A. (2001) Mol. Genet. Genom., 265, 405–413.

    Article  CAS  Google Scholar 

  8. Akopov, S. B., Ruda, V. M., Batrak, V. V., Vetchinova, A. S., Chernov, I. P., Nikolaev, L. G., Bode, J., and Sverdlov, E. D. (2006) Mamm. Genome, 17, 1042–1049.

    Article  PubMed  CAS  Google Scholar 

  9. Sambrook, J., and Russell, D. W. (2001) Molecular Cloning. A Laboratory Manual, 3rd Edn., CSHL Press, Cold Spring Harbor.

    Google Scholar 

  10. Tybulewicz, V. L., Crawford, C. E., Jackson, P. K., Bronson, R. T., and Mulligan, R. C. (1991) Cell, 65, 1153–1163.

    Article  PubMed  CAS  Google Scholar 

  11. Ruda, V. M., Akopov, S. B., Trubetskoy, D. O., Manuylov, N. L., Vetchinova, A. S., Zavalova, L. L., Nikolaev, L. G., and Sverdlov, E. D. (2004) Virus Res., 104, 11–16.

    Article  PubMed  CAS  Google Scholar 

  12. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W., and Lipman, D. J. (1997) Nucleic Acids Res., 25, 3389–3402.

    Article  PubMed  CAS  Google Scholar 

  13. Kuhn, R. M., Karolchik, D., Zweig, A. S., Trumbower, H., Thomas, D. J., Thakkapallayil, A., Sugnet, C. W., Stanke, M., Smith, K. E., Siepel, A., et al. (2007) Nucleic Acids Res., 35, D668–673.

    Article  PubMed  CAS  Google Scholar 

  14. Kellum, R., and Schedl, P. (1992) Mol. Cell. Biol., 12, 2424–2431.

    PubMed  CAS  Google Scholar 

  15. Borrelli, E., Heyman, R., Hsi, M., and Evans, R. M. (1988) Proc. Natl. Acad. Sci. USA, 85, 7572–7576.

    Article  PubMed  CAS  Google Scholar 

  16. Brand, A. H., Breeden, L., Abraham, J., Sternglanz, R., and Nasmyth, K. (1985) Cell, 41, 41–48.

    Article  PubMed  CAS  Google Scholar 

  17. Laimins, L., Holmgren-Konig, M., and Khoury, G. (1986) Proc. Natl. Acad. Sci. USA, 83, 3151–3155.

    Article  PubMed  CAS  Google Scholar 

  18. Chernov, I., Stukacheva, E., Akopov, S., Didych, D., Nikolaev, L., and Sverdlov, E. (2008) Biotechniques, 44, 775–784.

    Article  PubMed  CAS  Google Scholar 

  19. Akasaka, K., Nishimura, A., Takata, K., Mitsunaga, K., Mibuka, F., Ueda, H., Hirose, S., Tsutsui, K., and Shimada, H. (1999) Cell. Mol. Biol. (Noisy-le-grand), 45, 555–565.

    CAS  Google Scholar 

  20. Bi, X., and Broach, J. R. (1999) Genes Dev., 13, 1089–1101.

    Article  PubMed  CAS  Google Scholar 

  21. Gruzdeva, N., Kyrchanova, O., Parshikov, A., Kullyev, A., and Georgiev, P. (2005) Mol. Cell. Biol., 25, 3682–3689.

    Article  PubMed  CAS  Google Scholar 

  22. Belozerov, V. E., Majumder, P., Shen, P., and Cai, H. N. (2003) EMBO J., 22, 3113–3121.

    Article  PubMed  CAS  Google Scholar 

  23. Yannaki, E., Tubb, J., Aker, M., Stamatoyannopoulos, G., and Emery, D. W. (2002) Mol. Ther., 5, 589–598.

    Article  PubMed  CAS  Google Scholar 

  24. Zhou, J., Barolo, S., Szymanski, P., and Levine, M. (1996) Genes Dev., 10, 3195–3201.

    Article  PubMed  CAS  Google Scholar 

  25. Kuhn, E. J., Viering, M. M., Rhodes, K. M., and Geyer, P. K. (2003) EMBO J., 22, 2463–2471.

    Article  PubMed  CAS  Google Scholar 

  26. Maksimenko, O., Golovnin, A., and Georgiev, P. (2008) Mol. Cell. Biol., 28, 5469–5477.

    Article  PubMed  CAS  Google Scholar 

  27. Mongelard, F., and Corces, V. G. (2001) Nat. Struct. Biol., 8, 192–194.

    Article  PubMed  CAS  Google Scholar 

  28. Kyrchanova, O., Toshchakov, S., Parshikov, A., and Georgiev, P. (2007) Mol. Cell. Biol., 27, 3035–3043.

    Article  PubMed  CAS  Google Scholar 

  29. Recillas-Targa, F., Pikaart, M. J., Burgess-Beusse, B., Bell, A. C., Litt, M. D., West, A. G., Gaszner, M., and Felsenfeld, G. (2002) Proc. Natl. Acad. Sci. USA, 99, 6883–6888.

    Article  PubMed  CAS  Google Scholar 

  30. Brown, A. J., Goldsworthy, S. M., Barnes, A. A., Eilert, M. M., Tcheang, L., Daniels, D., Muir, A. I., Wigglesworth, M. J., Kinghorn, I., Fraser, N. J., et al. (2003) J. Biol. Chem., 278, 11312–11319.

    Article  PubMed  CAS  Google Scholar 

  31. Bazzi, H., Fantauzzo, K. A., Richardson, G. D., Jahoda, C. A., and Christiano, A. M. (2007) Dev. Dyn., 236, 961–970.

    Article  PubMed  CAS  Google Scholar 

  32. Herrmann, M., Selige, J., Raffael, S., Sachs, G., Brambilla, A., and Klein, T. (2007) Scand. J. Gastroenterol., 42, 1275–1288.

    Article  PubMed  CAS  Google Scholar 

  33. Chalaya, T. V., Akopov, S. B., Nikolaev, L. G., and Sverdlov, E. D. (2006) Biochemistry (Moscow), 71, 294–299.

    Article  CAS  Google Scholar 

  34. Takaki, R., Watson, S. R., and Lanier, L. L. (2006) Immunol. Rev., 214, 118–129.

    Article  PubMed  CAS  Google Scholar 

  35. Wu, J., Song, Y., Bakker, A. B., Bauer, S., Spies, T., Lanier, L. L., and Phillips, J. H. (1999) Science, 285, 730–732.

    Article  PubMed  CAS  Google Scholar 

  36. Kim, T. W., Wu, K., Xu, J. L., McAuliffe, G., Tanzi, R. E., Wasco, W., and Black, I. B. (1995) Brain Res. Mol. Brain Res., 32, 36–44.

    Article  PubMed  CAS  Google Scholar 

  37. Morimura, N., Inoue, T., Katayama, K., and Aruga, J. (2006) Gene, 380, 72–83.

    Article  PubMed  CAS  Google Scholar 

  38. Bell, A. C., West, A. G., and Felsenfeld, G. (2001) Science, 291, 447–450.

    Article  PubMed  CAS  Google Scholar 

  39. Willoughby, D. A., Vilalta, A., and Oshima, R. G. (2000) J. Biol. Chem., 275, 759–768.

    Article  PubMed  CAS  Google Scholar 

  40. Ruiz-Narvaez, E. A., and Campos, H. (2008) J. Hum. Genet., 53, 247–253.

    Article  PubMed  CAS  Google Scholar 

  41. Arnold, R., Maueler, W., Bassili, G., Lutz, M., Burke, L., Epplen, T. J., and Renkawitz, R. (2000) Gene, 253, 209–214.

    Article  PubMed  CAS  Google Scholar 

  42. Chernov, I., Akopov, S., and Nikolaev, L. (2004) Russ. J. Bioorg. Chem., 30, 1–11.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997, Moscow, Russia

    D. A. Didych, S. B. Akopov, E. V. Snezhkov, N. V. Skaptsova, L. G. Nikolaev & E. D. Sverdlov

Authors
  1. D. A. Didych
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. B. Akopov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. V. Snezhkov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. V. Skaptsova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. L. G. Nikolaev
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. E. D. Sverdlov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. B. Akopov.

Additional information

Original Russian Text © D. A. Didych, S. B. Akopov, E. V. Snezhkov, N. V. Skaptsova, L. G. Nikolaev, E. D. Sverdlov, 2009, published in Biokhimiya, 2009, Vol. 74, No. 7, pp. 899–905.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM08-387, June 7, 2009.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Didych, D.A., Akopov, S.B., Snezhkov, E.V. et al. Identification and mapping of ten new potential insulators in the FXYD5-COX7A1 region of human chromosome 19q13.12. Biochemistry Moscow 74, 728–733 (2009). https://doi.org/10.1134/S0006297909070049

Download citation

  • Received:

  • Revised:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0006297909070049

Key words

Search

Navigation