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Optimization of Metabolite Profiling for Black Medick (Medicago lupulina) and Peas (Pisum sativum)

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Abstract

Metabolic profiling is a key approach in current basic and applied research in biology. Comparative analysis of different metabolite extraction methods for pea (P. sativum) and black medick (M. lupulina) made it possible to find the optimal conditions for metabolite extraction and subsequent detection by gas chromatography coupled with mass spectrometry. The optimized method was shown to be reliable for assessment of the organ and species metabolic profiles for roots and leaves in pea and black medick plants.

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Authors and Affiliations

  1. St. Petersburg State University, St. Petersburg, 199034, Russia

    R. K. Puzanskiy, V. V. Yemelyanov, M. S. Kliukova, O. Yu. Shtark, A. P. Yurkov & M. F. Shishova

  2. Center for Molecular and Cell Technologies, St. Petersburg State University, St. Petersburg, 199034, Russia

    A. L. Shavarda

  3. All-Russia Research Institute for Agricultural Microbiology, St. Petersburg, 196608, Russia

    M. S. Kliukova, O. Yu. Shtark & A. P. Yurkov

Authors
  1. R. K. Puzanskiy
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  2. V. V. Yemelyanov
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  3. M. S. Kliukova
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  4. A. L. Shavarda
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  5. O. Yu. Shtark
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  6. A. P. Yurkov
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  7. M. F. Shishova
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Corresponding author

Correspondence to M. F. Shishova.

Additional information

Original Russian Text © R.K. Puzanskiy, V.V. Yemelyanov, M.S. Kliukova, A.L. Shavarda, O.Yu. Shtark, A.P. Yurkov, M.F. Shishova, 2018, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2018, Vol. 54, No. 4.

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Puzanskiy, R.K., Yemelyanov, V.V., Kliukova, M.S. et al. Optimization of Metabolite Profiling for Black Medick (Medicago lupulina) and Peas (Pisum sativum). Appl Biochem Microbiol 54, 442–448 (2018). https://doi.org/10.1134/S0003683818040129

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  • DOI: https://doi.org/10.1134/S0003683818040129

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