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Three-dimensional structure of the Arg32His mutant of the human tumor necrosis factor determined at 2.5 Å resolution from X-ray data for a twin crystal

  • Structures of Macromolecular Compounds
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Abstract

The three-dimensional structure of the Arg32His mutant of the human tumor necrosis factor (TNF-α) was established at 2.5 Å resolution by the molecular replacement method. The crystals of the mutant belong to sp. gr. R3. The specimen has a hemihedral twinning fraction of approximately one half with the twin law corresponding to an additional twofold axis along the a-or b-axis of the crystal lattice. The model analysis of interactions between functionally important loop 29–36 of the mutant and the receptors p55 and p75 was performed.

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References

  1. L. N. Shingarova, L. N. Sagaidak, R. L. Turetskaya, et al., Bioorg. Khim. 22, 243 (1996).

    Google Scholar 

  2. W. B. Coley, Am. J. Med. Sci. 105, 487 (1893).

    Google Scholar 

  3. L. J. Old, Science 230, 630 (1985).

    ADS  Google Scholar 

  4. E. A. Carswell, L. J. Old, R. L. Kassel, et al., Proc. Natl. Acad. Sci. USA 72, 3366 (1975).

    Google Scholar 

  5. B. Beutler, D. Greenwald, J. D. Hulmes, et al., Nature 316, 552 (1985).

    Article  Google Scholar 

  6. G. E. Nedwin, S. L. Naylor, A. Y. Sakaguchi, et al., Nucleic Acids Res. 13, 6361 (1985).

    Google Scholar 

  7. N. L. Paul and N. H. Ruddle, Annu. Rev. Immunol. 6, 407 (1988).

    Article  Google Scholar 

  8. B. Beutler, Tumor Necrosis Factors: The Molecules and Their Emerging Role in Medicine (Raven Press, New York, 1992).

    Google Scholar 

  9. H. Loetsher, Y.-C. E. Pan, H. W. Lahm, et al., Cell 61, 351 (1990).

    Google Scholar 

  10. T. J. Shall, M. Lewis, K. J. Koller, et al., Cell 61, 361 (1990).

    Google Scholar 

  11. C. A. Smith, T. Davis, D. Anderson, et al., Science 248, 1019 (1990).

    ADS  Google Scholar 

  12. C. Brakebusch, Y. Nophar, O. Kemper, et al., EMBO J. 11, 943 (1990).

    Google Scholar 

  13. L. A. Tartaglia, R. F. Weber, I. S. Figari, et al., Proc. Natl. Acad. Sci. USA 88, 9292 (1991).

    ADS  Google Scholar 

  14. L. A. Tartaglia and D. V. Goeddel, J. Biol. Chem. 267, 4304 (1992).

    Google Scholar 

  15. F. Mackay, H. Loetscher, G. Gehr, et al., J. Exp. Med. 177, 1277 (1992).

    Google Scholar 

  16. K. M. Thorp, C. Southern, I. N. Bird, and N. Mattews, Cytokine 4, 313 (1992).

    Article  Google Scholar 

  17. U. Trefzer, M. Brockhaus, H. Loetscher, et al., J. Invest. Dermatol. 97, 911 (1992).

    Google Scholar 

  18. S. Schutze, K. Potthof, T. Machleidt, et al., Cell 71, 765 (1992).

    Article  Google Scholar 

  19. C. H. W. Wong, J. H. Elwell, L. W. Oberley, and D. V. Goeddel, Cell 58, 923 (1989).

    Article  Google Scholar 

  20. M. J. Eck and S. R. Sprang, J. Biol. Chem. 264, 17 595 (1989).

    Google Scholar 

  21. M. J. Eck, M. Ultsch, E. Rinderknecht, et al., J. Biol. Chem. 267, 2119 (1992).

    Google Scholar 

  22. E. Y. Jones, D. I. Stuart, and N. P. C. Walker, Nature 338, 225 (1989).

    ADS  Google Scholar 

  23. S. S. Cha, J. S. Kim, H. S. Cho, et al., J. Biol. Chem. 273(4), 2153 (1998).

    Google Scholar 

  24. C. Reed, Z. Q. Fu, J. Wu, et al., Protein Eng. 10, 1101 (1997).

    Google Scholar 

  25. F. C. Bernstein, T. F. Koetzle, G. J. Williams, et al., J. Mol. Biol. 112, 535 (1977).

    Google Scholar 

  26. D. W. Banner, A. D’Arcy, W. Janes, et al., Cell 73, 431 (1993).

    Article  Google Scholar 

  27. D. Pennica, W. J. Kohr, B. M. Fendly, et al., Biochemistry 31, 1134 (1992).

    Article  Google Scholar 

  28. H. Loetsher, R. Gentz, M. Zulauf, et al., J. Biol. Chem. 229, 18 324 (1991).

  29. A. D’Arcy, D. W. Banner, W. Janes, et al., J. Mol. Biol. 229, 555 (1993).

    Google Scholar 

  30. M. R. Shalaby, A. Sundan, H. Laetsher, et al., J. Exp. Med. 172, 1517 (1990).

    Article  Google Scholar 

  31. R. Kircheis, J. Milleck, V. G. Korobko, et al., Immunology 76, 433 (1992).

    Google Scholar 

  32. C. R. Goh, C. S. Loh, and A. G. Porter, Protein Eng. 4, 385 (1991).

    Google Scholar 

  33. X. Van Ostade, J. Tavernier, T. Prange, and W. Fiers, EMBO J. 10, 827 (1991).

    Google Scholar 

  34. H. Loetscher, D. Stueber, D. Banner, et al., J. Biol. Chem. 268, 26 350 (1993).

  35. M. J. Eck, B. Beutler, G. Kuo, et al., J. Biol. Chem. 263, 12 816 (1988).

  36. B. W. Matthews, J. Mol. Biol. 33, 491 (1968).

    Google Scholar 

  37. A. T. Brunger, P. D. Adams, G. M. Clore, et al., Acta Crystallogr., Sect. D: Biol. Crystallogr. 54, 905 (1998).

    Google Scholar 

  38. T. O. Yeates, Methods Enzymol. 276, 344 (1997).

    Google Scholar 

  39. A. T. Brunger, X-PLOR (Version 3.1/3.851) Manual (Yale University, New Haven, 1992).

    Google Scholar 

  40. T. A. Jones, J. Appl. Crystallogr. 11, 268 (1978).

    Article  Google Scholar 

  41. P. V. Luzzati, Acta Crystallogr. 5, 802 (1952).

    Article  Google Scholar 

  42. Z.-Q. Fu, R. W. Harrison, C. Reed, et al., Protein Eng. 8, 1233 (1995).

    Google Scholar 

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Authors and Affiliations

  1. Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

    P. V. Afonin, A. V. Fokin, L. N. Shingarova, V. G. Korobko, I. N. Tsygannik, I. V. Artem’ev & V. Z. Pletnev

  2. Shubnikov Institute of Crystallography, Russian Academy of Sciences, Leninskii pr. 59, Moscow, 117333, Russia

    S. V. Pletnev

  3. Hauptman-Woodward Medical Research Institute, 73 High Street, Buffalo, New York, 14203-1196, USA

    W. Pangborn & W. L. Duax

Authors
  1. P. V. Afonin
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  2. A. V. Fokin
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  3. L. N. Shingarova
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  4. V. G. Korobko
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  5. I. N. Tsygannik
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  6. I. V. Artem’ev
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  7. S. V. Pletnev
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  8. W. Pangborn
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  10. V. Z. Pletnev
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__________

Translated from Kristallografiya, Vol. 47, No. 4, 2002, pp. 685–690.

Original Russian Text Copyright © 2002 by Afonin, Fokin, Shingarova, Korobko, Tsygannik, Artem’ev, S. Pletnev, Pangborn, Duax, V. Pletnev.

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Afonin, P.V., Fokin, A.V., Shingarova, L.N. et al. Three-dimensional structure of the Arg32His mutant of the human tumor necrosis factor determined at 2.5 Å resolution from X-ray data for a twin crystal. Crystallogr. Rep. 47, 629–634 (2002). https://doi.org/10.1134/1.1496062

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  • DOI: https://doi.org/10.1134/1.1496062

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