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Effect of ionizing radiation on multidrug resistance of human larynx cancer HEp-2 cells

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Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

Effects of ionizing radiation on multidrug resistance (MDR) of human larynx cancer HEp-2 cells have been studied. MDR was determined from sensitivity of HEp-2 cells to daunorubicin, taxol and vincristine in the absence and in the presence of MDR inhibitors (cyclosporin A and avermectin B1) and from the suppression by cyclosporin A of the rhodamine-123 release from HEp-2 cells. It was found that 8 and 16 h after irradiation (4 Gy), HEp-2 MDR was increased with a further return to the control level by the 24th hour after irradiation. The effect of irradiation was especially well-pronounced by 16 h for cells irradiated with 1 Gy and was manifested in enhanced release of rhodamine-123 and increased resistance of HEp-2 cells to vincristine. Besides, this effect depended on cell density, being at maximum at 80–100 × 103 cells/cm2. It is concluded that the observed dependence of HEp-2 MDR on ionizing radiation and cell density is a result of changes in intracellular content of reactive oxygen species.

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Authors and Affiliations

  1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia

    V. V. Shaposhnikova, A. F. Korystova, M. O. Emel’yanov, L. N. Kublik, A. A. Kudryavtsev, M. Kh. Levitman & Yu. N. Korystov

  2. Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia

    Yu. A. Kim

Authors
  1. V. V. Shaposhnikova
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  2. A. F. Korystova
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  3. M. O. Emel’yanov
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  4. L. N. Kublik
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  5. A. A. Kudryavtsev
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  6. M. Kh. Levitman
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  7. Yu. A. Kim
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  8. Yu. N. Korystov
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Correspondence to Yu. N. Korystov.

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Original Russian Text © V.V. Shaposhnikova, A.F. Korystova, M.O. Emel’yanov, L.N. Kublik, A.A. Kudryavtsev, M.Kh. Levitman, Yu.A. Kim, Yu.N. Korystov, 2007, published in Biologicheskie Membrany, 2007, Vol. 24, No. 5, pp. 413–420.

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Shaposhnikova, V.V., Korystova, A.F., Emel’yanov, M.O. et al. Effect of ionizing radiation on multidrug resistance of human larynx cancer HEp-2 cells. Biochem. Moscow Suppl. Ser. A 1, 294–300 (2007). https://doi.org/10.1134/S1990747807040046

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  • DOI: https://doi.org/10.1134/S1990747807040046

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