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Use of FITC as a Fluorescent Probe for Intracellular pH Measurement

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Abstract

Fluorescein Isothiocyanate (FITC) is widely used in biology and medicine as a fluorescent marker for labeling various proteins. Particularly fluorescence marking of antibodies could not be imagined without FITC. However, at the same time FITC displays pH-indicative properties. This paper evaluates the limits of the use of FITC as a pH indicator in biological material, namely, for intracellular and intraorganellar pH measurement.

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Authors and Affiliations

  1. Institute of Physiology, Czech Academy of Science, Vídeňská 1083, CZ-142 20, Prague 4, Czech Republic

    Edvard Lanz, Jan Slavík & Arnošt Kotyk

  2. Faculty of Natural Sciences, Charles University, Viničná 7, Prague2, Czech Republic

    Martin Gregor

Authors
  1. Edvard Lanz
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  2. Martin Gregor
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  3. Jan Slavík
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  4. Arnošt Kotyk
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Correspondence to Jan Slavík.

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Lanz, E., Gregor, M., Slavík, J. et al. Use of FITC as a Fluorescent Probe for Intracellular pH Measurement. Journal of Fluorescence 7, 317–319 (1997). https://doi.org/10.1023/A:1022586127784

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  • DOI: https://doi.org/10.1023/A:1022586127784

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