Abstract
The effect of four microalgal chlorophytes, Chlorella vulgaris,Scenedesmus quadricauda, Chlorococcum sp. and Ankistrodesmus convolutus, on the survival, larval development and adultbody size of the mosquito Aedes aegypti was investigated. The percentage mortality of larvae fed with C. vulgaris, Chlorococcum sp.or S. quadricauda after six days was 100%, 84% and 88%,respectively. Delayed pupation and body size reduction of the mosquitoesfed with C. vulgaris, Chlorococcum sp. and S. quadricaudawere observed. In contrast, larvae fed with A. convolutus werebigger than those fed with normal insectory feed. The study showed thatC. vulgaris, S. quadricauda and Chlorococcum sp. have potentialto be used as larvicidal agents.
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References
Abdeel-Hameed A, Kiviranta J (1993) Toxicity of the blue-green algae Oscillatoria agardhii to the mosquito Aedes aegypti and the shrimp Artemia salina. World J. Microbiol. Biotech. 10: 517-520.
Abdel-Hameed A, Kiviranta J, Sivonec K, Nienela S, Carlberg G (1994) Algae in mosquito breeding sites and the effectiveness of the mosquito larvicide Bacillus thuriengensis H14. World J. Microbiol. Biotech. 8: 151-159.
Amonkar SV (1969) Freshwater Algae and Their Metabolites as a Means of Biological Control of Mosquitoes. Unpublished PhD Dissertation, Department of Entomology, Univ. California, California, 102 pp.
Atkinson A, Gunning B, John P (1972) Sporopollenin in the cell wall of Chlorella and other algae: ultra-structure, chemistry and incorporation of 14acetate, studied in synchronous cultures. Planta, Berl. 1: 107.
Banerjee S, Rembold H (1993) Azadirachtin A interferes with control of serotonin pools in the neuroendocrine system of locusts. Naturwissenschften 9: 81-84.
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37: 911-917.
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analyt. Biochem. 72: 248-254.
Cheong WH (1965) Laboratory observations on comparative feeding preferences by Aedes from Kuantan and Singapore. Med. J. Malaya. 20: 73-74.
Chu WL, Phang SM, Goh SH (1995) Influence of carbon source on growth biochemical composition and pigmentation of Ankistrodesmus convolutus. J. appl. Phycol. 7: 59-64.
Clements AN (1963) The Physiology of Mosquitoes. Pergamon Press, Oxford: 234 pp.
Clements AN (1992) The Biology of Mosquitoes Vol 1. Development, nutrition and reproduction. Chapman and Hall, London, 640 pp.
Dadd RH (1975) Alkalinity within the midgut of mosquito larvae with alkaline active digestive enzymes. J. Insect Physiol. 21: 1847-1853.
Dhillon MS, Mulla MS (1981) Biological activity of the green algae Chlorella ellipsoidea against the immature mosquitoes. Mosq. News. 41: 368-372.
Dhillon MS, Mulla MS (1982) Impact of the green alga Chlorella ellipsoidea on the development and survival of mosquitoes breeding in cemetery vases. Envir. Ent. 11: 292-296.
Finney DJ (1989) Probit Analysis. Quant: Assays based on quantal response (Program modified from Tang Zhung-Ming). International Rice Research Institute, Los Baños, Philippines.
Harmis LD (1983) Increased adult size correlated with parity in Aedes triseriatus. Mosq. News. 43: 77-79.
Kochert AG (1978) Carbohydrate determination by the phenolsulphuric acid method. In Hellbust JA, Craigie JS (eds), Handbook of Phycological Methods-Phycological and biochemical methods. Cambridge Univ. Press, Cambridge, pp. 95-97.
Marten GG (1984) Impact of the copepod upon larval Aedes albopictus (Diptera: Culicidae). Bull. Soc. Vector Ecol. 9: 1-5.
Marten GG (1986) Phytoplankton management for mosquito control: the potential of indigestible green algae. J. trop. Med. Hyg. 89: 213-222.
Marten GG (1987) The potential of mosquito-indigestible phytoplankton for mosquito control. J. Am. Mosq. Contr. Ass. 3: 105-106.
Martin MM (1991) The evolution of cellulose digestion in insects. Phil. Trans. r. Soc. Lond. B. 333: 281-288.
McCombs SD (1980) Effect of differential nutrition of larvae on adult fitness ofAedes triseriatus. (Unpublished Master Thesis). University of Notre Dame, Notre Dame. 189 pp.
Phang SM, Chu WL (1999) University of Malaya Algae Culture Collection. Catalogue of Strains. University of Malaya, Kuala Lumpur, Malaysia, 77 pp.
Porter KG (1973) Selective Grazing and differential digestion of algae by zooplankton. Nature 244: 179-178.
Rashed SS, El-Ayouty YM (1991) Evaluation of certain algal species as biological-control agents against mosquito larvae. Bull. ent. Soc. Egypt Econ. Ser. 1991-1992 19: 1-7.
Rashed SS, El-Ayouty YM (1992) Impact of two unicellular algae on larval survival, development and adult body size of the mosquito Culex pipiens (Linn.). Bull. ent. Soc. Egypt Econ. Ser. 1991-1992 19: 243-247.
Renaud SM, Danny DL, Thinh LV (1994) Microalgae for use in tropical aquaculture 1: Gross chemical and fatty acid biocomposition of twelve species of microalgae from the Northern Territory, Australia. J. appl. Phycol. 6: 337-345.
Sagar SK, Sehgal SS (1997) Toxicity of neem seed coat extract against mosquitoes. Indian J. Ent. 59(2): 215-223.
Yang YJ, Davies DM (1971) Digestive enzymes in the excreta of Aedes aegypti larvae. J. Insect Physiol. 17: 2119-2123.
Zebitz CP (1986) Effect of three different neem seed kernel extracts and azadirachtin on larvae of different mosquito species. J. appl. Ent. 102: 422-463.
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Ahmad, R., Chu, WL., Lee, HL. et al. Effect of four chlorophytes on larval survival, development and adult body size of the mosquito Aedes aegypti . Journal of Applied Phycology 13, 369–374 (2001). https://doi.org/10.1023/A:1017966802600
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DOI: https://doi.org/10.1023/A:1017966802600