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Regulation of angiogenesis and endothelial cell motility by matrix-bound fibroblasts

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Abstract

This study sought to examine the effect of matrix-bound fibroblasts on angiogenesis and endothelial cell motility. Promotion of angiogenesis by matrix-bound fibroblasts was assessed using rat aortic ring and endothelial tube formation assays. Enhancement of human endothelial cell motility by matrix-bound fibroblasts was assessed using cytodex-2 bead and colloidal gold phagokinetic motility assays. Antibody to hepatocyte growth factor/scatter factor (HGF/SF) but not vascular endothelial growth factor (VEGf) decreased fibroblast-enhanced motility of endothelial cells. The promotion of tube formation by matrix-bound fibroblasts was neutralised with antibodies to HGF/SF and VEGf, both known promoters of angiogenesis. HGF/SF presence was detected by ELISA; whilst the presence of VEGf was detected by Western blotting. These data show that matrix-embedded fibroblasts regulate the motility of vascular endothelial cells and enhance angiogenesis, an effect partly attributed to production of angiogenesis-promoting cytokines HGF/SF and/or VEGf.

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Authors and Affiliations

  1. Wound Healing Research Unit, University of Wales College of Medicine, Cardiff, UK

    T.A. Martin & K.G. Harding

  2. Department of Surgery, University of Wales College of Medicine, Cardiff, UK

    W.G. Jiang

Authors
  1. T.A. Martin
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  2. K.G. Harding
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  3. W.G. Jiang
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Martin, T., Harding, K. & Jiang, W. Regulation of angiogenesis and endothelial cell motility by matrix-bound fibroblasts. Angiogenesis 3, 69–76 (1999). https://doi.org/10.1023/A:1009004212357

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  • DOI: https://doi.org/10.1023/A:1009004212357

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