Abstract
The differential hosts of Williams (1966) and the European Clubroot Differential (ECD) (Buczacki et al., 1975) have been used commonly to identify populations of Plasmodiophora brassicae, which causes clubroot disease in Brassica crops. However, some of these hosts showed intermediate and fluctuating scores to most populations from Japan. Therefore, these hosts could not be used to provide a clear classification in Japan. We have tried to clarify the genetic diversity in pathogenicity of P. brassicae in Japan using Japanese clubroot-resistant (CR) F1 hybrid (F1) cultivars and lines of Brassica rapa. The responses of some CR F1 cultivars were very clear. Four groups of field populations in Japan were recognized using the CR F1 cultivars. The clear response obtained here may depend largely on the genetic purity of the F1 cultivars. Moreover, it is possible to classify some of these Japanese populations into the same race using the Williams set and ECD 01 to ECD 05. The present differential hosts may be useful in the study of European populations of P. brassicae. The response of the differential hosts suggests that there are several major CR genes in B. rapa. It is suggested that pyramiding CR genes would be useful in breeding CR cultivars that can overcome the breakdown of the present CR cultivars of Chinese cabbage.
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References
Buczacki ST, Toxopeus H, Mattusch P, Johnston TD, Dixon GR and Hobolth LA (1975) Study of physiologic specialization in Plasmodiophora brassicae: Proposals for attempted rationalization through an international approach. Trans Br Mycol Soc 65: 295-303
Buczacki ST (1977) Root infections from single resting spores of Plasmodiophora brassicae. Br Mycol Soc 69: 328-329
Crute IR, Gray AR, Crisp P and Buczacki ST (1980) Variation in Plasmodiophora brassicae and resistance to clubroot disease in brassicas and allied crops — a critical review. Pl Breed Abst 50: 91-104
Crute IR and Pink DAC (1989) The characteristics and inheritance of resistance to clubroot in Brassica oleracea. Asp Applied Biol 23: 57-60
Figdore SS, Ferreira ME, Slocum MK and Williams PH (1993) Association of RFLP markers with trait loci affecting clubroot resistance and morphological characters in Brassica oleracea L. Euphytica 69: 33-44
Grandclement C and Thomas G (1996) Detection and analysis of QTLs based on RAPD markers for polygenic resistance to Plasmodiophora brassicae Woron in Brassica oleracea L. Theor Appl Genet 93: 86-90
Haji Tinggal S and Webster J (1981) Technique for single spore infection by Plasmodiophora brassicae. Trans Br Mycol Soc 76: 187-190
Jones DR, Ingram DS and Dixon GR (1982) Characterization of isolates derived from single resting spores of Plasmodiophora brassicae and studies of their interaction. Plant Pathol 31: 239-246
Karling JS (1968) The Plasmodiophorales (pp 239) Hafner Publ. Co., New York
Kuginuki Y, Yoshikawa H and Hida K (1994) Breeding of Chinese cabbage with clubroot resistance in Japan. In: ISHS Symposium on Brassicas, Abstracts of 9th Crucifer Genetics Workshop (pp 21) Lisbon
Kuginuki Y, Ajisaka H, Yui M, Yoshikawa H, Hida K and Hirai M (1997) RAPD markers linked to a clubroot-resistance locus in Brassica rapa L. Euphytica 98: 149-154
Landry BS, Hubert N, Crete R, Chiang MS, Lincoln SE and Etoh T (1992) A genetic map for Brassica oleracea based on RFLP markers detected with expressed DNA sequences and mapping of resistance genes to race 2 of Plasmodiophora brassicae (Woronin). Genome 35: 409-420
Naiki T (1987) Life cycle and control of Plasmodiophora brassicae, causing clubroot disease of cruciferous plants. Soil Micro 29: 23-38
Pink DAC, King GJ, Ockendon DJ, McLenaghan R and Lynn J (1994) Use of anther culture and molecular markers in breeding for resistance to Plasmodiophora brassicae. In: ISHS Symposium on Brassicas, Abstracts of 9th Crucifer Genetics Workshop (pp 15) Lisbon
Some A, Manzanares MJ, Laurens F, Baron F, Thomas G and Rouxel F (1996) Variation for virulence on Brassica napus L. amongst Plasmodiophora brassicae collections from France and derived single-spore isolates. Plant Pathology 45: 432-439
Voorrips RE (1995) Plasmodiophora brassicae: Aspects of pathogenesis and resistance in Brassica oleracea. Euphytica 83: 139-146
Voorrips RE, Jongerius MC and Kanne HJ (1997) Mapping of two genes for resistance to clubroot (Plasmodiophora brassicae) in a population of doubled haploid lines of Brassica oleracea by means of RFLP and AFLP markers. Theor Appl Genet 94: 75-82
Wilcoxon F (1945) Individual comparisons by ranking methods. Biometrics 1: 80-83
Williams PH (1966) A system for the determination of races of Plasmodiophora brassicae that infect cabbage and rutabaga. Phytopathology 56: 624-626
Yoshikawa H (1981) Breeding for clubroot resistance in Chinese cabbage. In: NS Talekar and TD Griggs (ed.) Chinese Cabbage (pp 405-413) Proceedings of the 1st International Symposium, Tsukuba, Japan
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Kuginuki, Y., Yoshikawa, H. & Hirai, M. Variation in Virulence of Plasmodiophora brassicae in Japan Tested with Clubroot-resistant Cultivars of Chinese Cabbage (Brassica rapa L. ssp. pekinensis). European Journal of Plant Pathology 105, 327–332 (1999). https://doi.org/10.1023/A:1008705413127
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DOI: https://doi.org/10.1023/A:1008705413127