Abstract
Pseudomonas fluorescens IP01 grown on isopropylbenzene (cumene) and Acinetobacter sp. 20B grown on dimethyl sulfide (DMS) degraded up to 90% and 25% of 1.5 mg trichloroethylene (TCE)/l, respectively. Escherichia coli harboring the DMS monooxygenase genes from strain 20B, the cumene dioxygenase genes from strain IP01 and both oxygenase genes, degraded up to 50%, 75% and 88% of 75 mg TCE/l, respectively. The growth rates of the E. coli recombinants remained nearly unaffected by TCE at 15 150 mg/l. Thus, the E. coli recombinants were indicated to degrade high concentrations of TCE efficiently at least up to 150 mg l−1.
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Adam W, Bialas J, Hadjiarapoglou L (1991) Chem. Ber. 124: 2377.
Alvarez-Cohen L, McCarty PL (1991) Appl. Environ. Microbiol. 57: 228–235.
Aoki H, Kimura T, Habe H, Yamane H, Kodama T, Omori T (1996) J. Ferment. Bioeng. 81: 187–196.
Ayobi PK, Harker AR (1998) Appl. Environ. Microbiol. 64: 4353–4356.
Bovicelli P, Lupatterlli P, Mincione E (1992) J. Org. Chem. 57: 2182–2184.
Fitch MW, Speitel GE Jr, Georgiou G (1996) Appl. Environ. Microbiol. 62: 1124–1128.
Freitas dos Santos LM, Livingston AG (1995) Biotechnol. Bioeng. 47: 82–89.
Furukawa K, Hirose J, Hayashida S, Nakamura K (1994) J. Bacteriol. 176: 2121–2123.
Heald S, Jenkins RO (1994) Appl. Environ. Microbiol. 60: 4634–4637.
Horinouchi M, Kasuga K, Nojiri H, Yamane H, Omori T (1997) FEMS Microbiol. Lett. 155: 99–105.
Jahng D, Wood TK (1994) Appl. Environ. Microbiol. 60: 2473–2482.
Little CD, Palumbo AV, Herbes SE, Lidstrom ME, Tyndall RL, Gilmer PJ (1988) Appl. Environ. Microbiol. 54: 951–956.
Nakajima T, Uchiyama H, Yagi O, Nakahara T (1992) Biosci. Biotech. Biochem. 56, 486–489.
Nelson MJK, Montgomery SO, Mahaffey WR, Pritchard PH (1987) Appl. Environ. Microbiol. 53: 949–954.
Newman LM, Wackett LP (1991) Appl. Environ. Microbiol. 57: 2399–2402.
Oldenhuis R, Oedzes JY, van der Waarde JJ, Janssen DB (1991) Appl. Environ. Microbiol. 57: 7–14.
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning, 2nd ed., A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
Speitel GE Jr, Thompson RC, Weissman D (1993) Water Res. 27: 15–24.
Tsien HC, Brusseau GA, Hanson RS, Wackett LP (1989) Appl. Environ. Microbiol. 55: 3155–3161.
Wackett LP, Gibson DT (1988) Appl. Environ. Microbiol. 54: 1703–1708.
Wackett LP, Householder SR (1989) Appl. Environ. Microbiol. 55, 2723–2725.
Yanish-Perron C, Vieira J, Messing J (1985) Gene 33: 103–119.
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Takami, W., Horinouchi, M., Nojiri, H. et al. Evaluation of trichloroethylene degradation by E. coli transformed with dimethyl sulfide monooxygenase genes and/or cumene dioxygenase genes. Biotechnology Letters 21, 259–264 (1999). https://doi.org/10.1023/A:1005404931086
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DOI: https://doi.org/10.1023/A:1005404931086