Abstract
In this study, the quantification of scopolamine from Brugmansia candida normal roots is described. The roots were obtained and cultured in half salts strength Schenk and Hildebrandt (1/2 SH) liquid medium plus 30 g l−1 sucrose and supplemented with 3 mg l−1 of α-naphthaleneacetic acid. The quantitative determination of scopolamine was carried out by HPLC. The maximum scopolamine production in Brugmansia candida normal root culture was 3.94±0.01 mg g−1 dry weight obtained at 24th day of culture.
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Niño, J., Gallego, C.M., Correa, Y.M. et al. Production of scopolamine by normal root cultures of Brugmansia candida . Plant Cell, Tissue and Organ Culture 74, 289–291 (2003). https://doi.org/10.1023/A:1024028923849
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DOI: https://doi.org/10.1023/A:1024028923849