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The tensile strength of laser welding of an incision in the keratinized oral mucosa of rabbits in vivo

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Abstract

Introduction

In this study, coagulation of the blood at the wound side is evoked using a 980-nm-wavelength diode laser. The laser is used to approximate incision wound edges to be compared with the healing of the sutured incision. Clinical observations, histological slides, and tensile strength measurement are performed to assess the quality of healing.

Material and methods

The study is conducted on 24 albino rabbits; an intraoral incision in the hard palate is done on both sides, right and left, for each rabbit. One side serves as a control group where the incision is sutured, while the other side’s incision is welded by a 980-nm-wavelength laser. The laser is operated in continuous wave mode (CW) 20 W/cm2 power density for 27 s exposure time.

Results

Clinically, there was some loss of tissue details (smooth feature) on the welded side in comparison with the conventionally treated side. Histopathologically, healing of the epithelial layer was perfect, while the submucosal layer showed loss of angiogenesis and loose connective tissue replacing the normal structure. The tensile strength measurement shows comparable results for the welded incision and the sutured incision; moreover, the immediate and first-day tensile strength of the welded incision shows superior results to the sutured incision.

Recommendation

Further studies are needed to monitor histologically the healing after laser welding and measure the development in the tensile strength to assess the validity of the 980-nm-wavelength laser beam as a tool in tissue welding.

Conclusion

Nine hundred eighty-nanometer-wavelength laser beam is a suitable tool for welding incisions in the oral cavity at areas subjected to stress, such as a socket after tooth extraction.

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Authors and Affiliations

  1. Oral Surgery Branch, College of Dentistry, University of Kirkuk, Kirkuk, Iraq

    Balsam M. Mirdan

  2. Azadi Teaching Hospital, Kirkuk, Iraq

    Suzan Naji

  3. Biophotonics Laboratory, Institute of Biomedical Engineering, Bogazici University, Istanbul, Turkey

    Ayșa Sena Kabas Sarp & Murat Gulsoy

Authors
  1. Balsam M. Mirdan
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  2. Suzan Naji
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  3. Ayșa Sena Kabas Sarp
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  4. Murat Gulsoy
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Corresponding author

Correspondence to Balsam M. Mirdan.

Ethics declarations

This experiment on the rabbits was conducted under a protocol approved by the Institutional Animal Research and Care Ethics Committee at Bogazici University.

Conflict of interest

The research was perform on rabbits to create surgical incisions using surgical scalpel at keratinized oral mucosa of the hard palate. Each incision was welded using diode laser 980nm instue. Each sample was monitored clinically and studied histologically during the healing phases in addition to the tensile strength measurement which was performed. All the previously mentioned teste were compared with conventional sutured technique which is used now day.

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Mirdan, B.M., Naji, S., Sarp, A.S.K. et al. The tensile strength of laser welding of an incision in the keratinized oral mucosa of rabbits in vivo. Laser Dent Sci 3, 183–189 (2019). https://doi.org/10.1007/s41547-019-00059-6

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  • DOI: https://doi.org/10.1007/s41547-019-00059-6

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