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The pre-mir-27a variant rs895819 may contribute to type 2 diabetes mellitus susceptibility in an Iranian cohort

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Abstract

Purpose

The study was aimed at investigating the association between hsa-mir-27a polymorphism rs895819 (T/C) and type 2 diabetes mellitus (T2DM) susceptibility in a large Iranian cohort.

Methods

In this case–control study, the investigated population consisted of T2DM patients (n = 204) and sex- and age-matched controls (n = 209). We used the polymerase chain reaction and restriction fragment length polymorphism (PCR–RFLP) for genotyping.

Results

We observed significant differences between T2DM patients and controls for weight (p = 0.002), BMI (p < 0.001), systolic blood pressure (p < 0.001), diastolic blood pressure (p < 0.001), fasting plasma glucose (p < 0.001), triglyceride (p = 0.004) and LDL cholesterol (p = 0.051). Moreover, we found that genotype distributions were significantly different between groups (p < 0.05) and that the rs895819-C allele is more frequent in controls (p = 0.030, OR = 0.72, 95 % CI 0.53–0.97).

Conclusion

Our study shows that rs895819 in hsa-mir-27a is associated with T2DM susceptibility and that the C allele conveyed a protective role against T2DM. Larger multicentric and specific functional studies will be necessary to obtain a deeper comprehension of the role of rs895819 and hsa-mir-27a and how they are involved in the development of diabetes.

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Acknowledgments

We thank the Shahid Beheshti University of Medical Sciences (Grant Number 1393-1-91-13285) and the Italian Ministry of Health (RC-2015 and RC-2016) for financial support.

Funding

This study was granted by Shahid Beheshti University of Medical Sciences (Grant Number: 1393-1-91-13285) and the Italian Ministry of Health (RC-2015 and RC-2016).

Author information

Authors and Affiliations

  1. Medical Genetics Department, School of Medicine, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    H. Ghaedi, F. Shokri, A. Movafagh, R. Mirfakhraie & M. D. Omrani

  2. Medical Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran

    M. Tabasinezhad

  3. Clinical Biochemistry Department, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    B. Alipoor

  4. Gene Expression - Microarrays Laboratory, Bambino Gesù Children’s Hospital-IRCCS, Viale di San Paolo 15, 00146, Rome, Italy

    A. Masotti

Authors
  1. H. Ghaedi
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  2. M. Tabasinezhad
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  3. B. Alipoor
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  4. F. Shokri
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  5. A. Movafagh
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  6. R. Mirfakhraie
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  7. M. D. Omrani
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  8. A. Masotti
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Corresponding authors

Correspondence to M. D. Omrani or A. Masotti.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in the study involving human participants were in accordance with the ethical standards of the institutional research committee and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Cite this article

Ghaedi, H., Tabasinezhad, M., Alipoor, B. et al. The pre-mir-27a variant rs895819 may contribute to type 2 diabetes mellitus susceptibility in an Iranian cohort. J Endocrinol Invest 39, 1187–1193 (2016). https://doi.org/10.1007/s40618-016-0499-4

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  • DOI: https://doi.org/10.1007/s40618-016-0499-4

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