Abstract
Aims
To quantitatively identify Bifidobacterium, S. wiggsiae and S. mutans in plaque samples obtained from children with severe-ECC and caries-free groups and to analyze their association with caries-related factors retrieved from the questionnaire in each group.
Study design
To establish the 2 study groups, clinical examination in 122 Thai children, aged 2–5 years, recorded decayed, missing and filled teeth scores (dmft), in addition to plaque and gingival indices. Sixty one children in the caries-free group and 61 in the S-ECC group were identified. A questionnaire was used to assess the parent’s attitudes and behavior regarding the child’s oral hygiene care and diet.
Methods
Pooled overnight supra gingival plaque was collected from each child using a sterile toothpick, released in 1 ml of TE buffer, transported on ice to the Laboratory and stored at – 20 °C. DNA was extracted from the plaque based on enzymatic lysis and quantitative real-time PCR using fluorescent dye (SYBR green) in addition to Agarose gel electrophoresis were performed. All laboratory and retrieved from the questionnaire data per child were recorded and statistically analysed.
Results
S. wiggsiae (p < 0.005) and S. mutans (p < 0.001) were higher in the S-ECC group. Bifidobacterium, S. mutans, and S. wiggsiae were associated with the dmft score and gingival index (p < 0.001). The dmft scores of children who detected only S. mutans were significantly lower than the dmft scores of children who detected two bacteria; S. mutans + S. wiggsiae (p = 0.028), S. mutans + Bifidobacterium (p = 0.026), and three bacteria; S. mutans + Bifidobacterium + S. wiggsiae (p = 0.007). Children who found all three bacteria (Bi + Sm + Sw) had the highest dmft scores, followed by children who had two bacteria (Bi + Sw, or Bi + Sm, or Sw + Sm). The guardians’ education levels, occupations, household income, prolonged bottle feeding, taking of water after bottle or breast feeding, eating sugar-coated crackers or bread with sweetened cream, and premature birth were the factors that related to S-ECC.
Conclusion
Levels of S. wiggsiae and S. mutans, guardian’s education, family economics, prolonged bottle feeding, eating high sugar-containing snacks and premature birth were associated with S-ECC.
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References
American Academy of Pediatric Dentistry. Policy on early childhood caries (ECC): classifications, consequences, and preventive strategies. Pediatr Dent. 2016;38(6):52–4.
Anil S, Anand PS. Early childhood caries: prevalence, risk factors, and prevention. Front Pediatr. 2017;5:157. https://doi.org/10.3389/fped.2017.00157.
Becker MR, Paster BJ, Leys EJ, Moeschberger ML, Kenyon SG, Galvin JL, et al. Molecular analysis of bacterial species associated with childhood caries. J Clinicalmicrobiol. 2002;40(3):1001–9. https://doi.org/10.1128/JCM.40.3.1001-1009.2002.
Beighton D, Adamson A, Rugg-Gunn A. Associations between dietary intake, dental cariesexperience and salivary bacterial levels in 12-year-old English schoolchildren. Arch Oral Biol. 1996;41(3):271–80. https://doi.org/10.1016/0003-9969(96)84555-9.
Beighton D, Gilbert SC, Clark D, et al. Isolation and Identification of Bifidobacteriaceae from Human Saliva. Appl Environ Microbiol. 2008;74(20):6457–60. https://doi.org/10.1128/AEM.00895-08.
Carmona LE, Reyes N, González F Polymerase chain reaction for detection of Streptococcus mutans and Streptococcus sobrinus in dental plaque of children from Cartagena, Colombia. Colombia Médica. 2011;42:430–7. https://doi.org/10.25100/cm.v42i4.943
Carr G, Alexander A, Nguyen L, Kingsley K. Oral site specific sampling reveals differential location for scardovia wiggsiae. Microbiol Res J Int. 2020. https://doi.org/10.9734/mrji/2020/v30i130189.
Chandna P, Srivastava N, Sharma A, Sharma V, Gupta N, Adlakha V. Isolation of Scardovia wiggsiae using real-time polymerase chain reaction from the saliva of children with early childhood caries. J Indian Soc Pedodont Prevent Dentistry. 2018;36:290. https://doi.org/10.4103/JISPPD.JISPPD_225_17.
Chanpum P, Duangthip D, Trairatvorakul C, Songsiripradubboon S. Early childhood caries and its associated factors among 9- to 18-month old exclusively breastfed childrenin Thailand: a cross-sectional study. Int J Environ Res Public Health. 2020;17:3194. https://doi.org/10.3390/ijerph17093194.
Choi EJ, Lee SH, Kim YJ. Quantitative real-time polymerase chain reaction for Streptococcus mutans and Streptococcus sobrinus in dental plaque samples and its association with early childhood caries. Int J Paediat Dentist. 2009;19(2):141–7. https://doi.org/10.1111/j.1365-263X.2008.00942.x.
Colombo NH, Kreling PF, Ribas LFF, Pereira JA, Kressirer CA, Klein MI, et al. Quantitative assessment of salivary oral bacteria according to the severity of dental caries in childhood. Arch Oral Biol. 2017;83:282–8. https://doi.org/10.1016/j.archoralbio.2017.08.006.
de Matos BM, Brighenti FL, Do T, Beighton D, Koga-Ito CY. Acidogenicity of dual-species biofilms of bifidobacteria and Streptococcus mutans. Clin Oral Invest. 2017;21(5):1769–76. https://doi.org/10.1007/s00784-016-1958-1.
Ghimire N, Rao A. Comparative evaluation of the influence of television advertisements on children and caries prevalence. Glob Health Action. 2013;6:20066. https://doi.org/10.3402/gha.v6i0.20066.
Greene JG, Vermillion JR. The simplified oral hygiene index. J Am Dental Assoc 1964;68(1):7–13. https://doi.org/10.14219/jada.archive.1964.0034
Hallett KB, O’Rourke PK. Caries experience in preschool children referred for specialist dental care in hospital. Aust Dent J. 2006;51(2):124–9. https://doi.org/10.1111/j.1834-7819.2006.tb00415.x.
Haukioja A, Yli-Knuuttila H, Loimaranta V, Kari K, Ouwehand AC, Meurman JH, et al. Oral adhesion and survival of probiotic and other lactobacilli and bifidobacteria in vitro. Oral Microbiol Immunol. 2006;21(5):326–32. https://doi.org/10.1111/j.1399-302X.2006.00299.x.
Henne K, Rheinberg A, Melzer-Krick B, Conrads G. Aciduric microbial taxa including Scardovia wiggsiae and Bifidobacterium spp in caries and caries free subjects. Anaerobe. 2015;35(Pt A):60–5. https://doi.org/10.1016/j.anaerobe.2015.04.011.
Hughes CV, Dahlan M, Papadopolou E, Loo CY, Pradhan NS, Lu SC, et al. Aciduric microbiota and mutans streptococci in severe and recurrent severe early childhood caries. Pediatr Dent. 2012;34(2):e16-23.
Ismail AI, Sohn W, Lim S, Willem JM. Predictors of dental caries progression in primary teeth. J Dent Res. 2009;88(3):270–5. https://doi.org/10.1177/0022034508331011.
Jian W, Dong X. Transfer of Bifidobacterium inopinatum and Bifidobacterium denticolens to Scardovia inopinata gen. nov., comb nov., and Parascardovia denticolens gen. nov., comb nov., respectively. Int J Syst Evolut Microbiol. 2002;52:809–12. https://doi.org/10.1099/00207713-52-3-809.
Kameda M, Abiko Y, Washio J, Tanner ACR, Kressirer CA, Mizoguchi I, et al. Sugar metabolism of Scardovia wiggsiae, a novel caries-associated bacterium. Front Microbiol. 2020. https://doi.org/10.3389/fmicb.2020.00479.
Kanasi E, Dewhirst FE, Chalmers NI, Kent R Jr, Moore A, Hughes CV, et al. Clonal analysis of the microbiota of severe early childhood caries. Caries Res. 2010;44(5):485–97. https://doi.org/10.1159/000320158.
Li Y, Tanner A. Effect of antimicrobial interventions on the oral microbiota associated with early childhood caries. Pediatr Dent. 2015;37(3):226–44.
Lobene R. A modified gingival index for use in clinical trials. Clin prevent Dent. 1986;8:3–6.
Manome A, Abiko Y, Kawashima J, Washio J, Fukumoto S, Takahashi N. Acidogenic Potential of Oral Bifidobacterium and Its High Fluoride Tolerance. Front Microbiol. 2019;10:1099. https://doi.org/10.3389/fmicb.2019.01099
Mantzourani M, Gilbert SC, Sulong HN, Sheehy EC, Tank S, Fenlon M, et al. The isolation of bifidobacteria from occlusal carious lesions in children and adults. Caries Res. 2009;43(4):308–13. https://doi.org/10.1159/000222659.
Matsuki T, Watanabe K, Fujimoto J, Kado Y, Takada T, Matsumoto K, et al. Quantitative PCR with 16S rRNA-gene-targeted species-specific primers for analysis of human intestinal bifidobacteria. Appl Environ Microbiol. 2004;70(1):167–73. https://doi.org/10.1128/AEM.70.1.167-173.2004.
Mitrakul K, Akarapipatkul B, Thammachat P. Quantitative Analysis of Streptococcusmutans, Streptococcus sobrinus and Streptococcus sanguinis and their association with Early Childhood Caries. J Clin Diagn Res. 2019;13(10):17–21. https://doi.org/10.7860/JCDR/2020/43086.13513.
Mitrakul K, Chan.vitan S, Jeamset A, Vongsawan K. Quantitative analysis of S. mutans, Lactobacillus and Bifidobacterium found in initial and mature plaques in Thai children with early childhood caries. Eur Arch Paediat Dentist. 2017;18(4):251–61. https://doi.org/10.1007/s40368-017-0295-7.
Modesto M, Biavati B, Mattarelli P. Occurrence of the family bifidobacteriaceae in human dental caries and plaque. Caries Res. 2006;40(3):271–6. https://doi.org/10.1159/000092237.
Nair S, Kumar VS, Krishnan R, Rajan P. A comparative evaluation of bifidobacteria levels in early childhood caries and severe early childhood caries. J Pharm Bioall Sci 2017;9, Suppl S1:82–4 https://doi.org/10.4103/jpbs.JPBS_75_17
Palmer CA, Kent R Jr, Loo CY, Hughes CV, Stutius E, Pradhan N, et al. Diet and caries associated bacteria in severe early childhood caries. J Dent Res. 2010;89(11):1224–9. https://doi.org/10.1177/0022034510376543.
Pierce A, Singh S, Lee J, Grant C, Cruz de Jesus V, Schroth RJ. The Burden of Early Childhood Caries in Canadian Children and Associated Risk Factors. Front Public Health. 2019;7:328. https://doi.org/10.3389/fpubh.2019.00328
Rugg-Gunn AJ, Woodward M, editors. Review of the aetiology of Early Childhood Caries 2017
Rusali R, Hamali N, Razi F, Mustafa N, Harun N, Azwani N. Early Childhood Feeding Practices and Its Association with Early Childhood Caries. 2019:801–4. https://doi.org/10.12691/jfnr-7-11-7
Shi L, Jia J, Li C, Zhao C, Li T, Shi H, et al. Relationship between preterm, low birth weight and early childhood caries: a meta-analysis of the case-control and cross-sectional study. 2020. Biosci Rep. https://doi.org/10.1042/BSR20200870.
Singla D, Sharma A, Sachdev V, Chopra R. Distribution of Streptococcus mutans and Streptococcus sobrinus in Dental Plaque of Indian Pre-School Children Using PCR and SB-20M Agar Medium. J Clin Diagn Res. 2016;10(11):Zc60-zc3. https://doi.org/10.7860/JCDR/2016/19256.8909
Sinsimer D, Leekha S, Park S, Marras SA, Koreen L, Willey B, et al. Use of a multiplex molecular beacon platform for rapid detection of methicillin and vancomycin resistance in Staphylococcus aureus. J Clin Microbiol. 2005;43(9):4585–91. https://doi.org/10.1128/JCM.43.9.4585-4591.2005.
Tanner AC, Mathney JM, Kent RL, Chalmers NI, Hughes CV, Loo CY, et al. Cultivable anaerobic microbiota of severe early childhood caries. J Clin Microbiol. 2011;49(4):1464–74. https://doi.org/10.1128/JCM.02427-10.
Tanner AC, Kressirer CA, Faller LL. Understanding caries from the oral microbiome perspective. J Calif Dent Assoc. 2016;44(7):437–46.
Tanzer JM, Livingston J, Thompson AM. The microbiology of primary dental caries in humans. J Dent Educ. 2001;65(10):1028–37.
The 8th national oral health survey in 2017, Thailand. Bangkok: Ministry of Public Health.
Topcuoglu N, Aktoren O. Scardovia wiggsiae and the other microorganisms in severe early childhood caries. J Dentist Oral Care Med. 2017. https://doi.org/10.1128/JCM.02427-10.
Torlakovic L, Klepac-Ceraj V, Ogaard B, Cotton SL, Paster BJ, Olsen I. Microbial community succession on developing lesions on human enamel. J Oral Microbiol. 2012. https://doi.org/10.3402/jom.v4i0.16125.
Vacharaksa A, Suvansopee P, Opaswanich N, Sukarawan W. PCR detection of Scardovia in combination with Streptococcus mutans for early childhood caries-risk prediction. Eur J Oral Sci. 2015. https://doi.org/10.1111/eos.12208.
Valdez RM, Dos Santos VR, Caiaffa KS, Danelon M, Arthur RA, Negrini TC, et al. Comparative in vitro investigation of the cariogenic potential of bifidobacteria. Arch Oral Biol. 2016;71:97–103. https://doi.org/10.1016/j.archoralbio.2016.07.005.
Yano A, Kaneko N, Ida H, Yamaguchi T, Hanada N. Real-time PCR for quantification of Streptococcus mutans. FEMS Microbiol Lett. 2002;217(1):23–30. https://doi.org/10.1111/j.1574-6968.2002.tb11451.x.
Zeng X, Sheiham A, Sabbah W. The association between dental caries and television viewing mong Chinese adolescents in Guangxi. China BMC Oral Health. 2014;14:138. https://doi.org/10.1186/1472-6831-14-138.
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Tantikalchan, S., Mitrakul, K. Association between Bifidobacterium and Scardovia Wiggsiae and caries-related factors in severe early childhood caries and caries-free Thai children: a quantitative real-time PCR analysis and a questionnaire cross-sectional study. Eur Arch Paediatr Dent 23, 437–447 (2022). https://doi.org/10.1007/s40368-022-00702-0
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DOI: https://doi.org/10.1007/s40368-022-00702-0