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Adhesion of three types of fissure sealant in saliva-contaminated and noncontaminated conditions: an in vitro study

  • Original Scientific Article
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European Archives of Paediatric Dentistry Aims and scope Submit manuscript

Abstract

Purpose

To evaluate the proportion of microleakage (PM), shear bond strength (SBS), and the fissure sealant (FS) interface by scanning electron microscopy (SEM) in three kinds of FS when the enamel surfaces were contaminated with saliva.

Methods

198 sound third molar teeth were randomly divided into three pretreatment condition groups (n = 66): dry, saliva contamination removed by cotton pellet, or saliva removed by air-drying. A resin-based FS (Clinpro™), amorphous calcium phosphate-containing FS (Aegis®), or glass ionomer-based FS (Fuji Triage®) was applied on the treated enamel, and PM and SBS were assessed. Two specimens from each group were observed with SEM. p values < 0.05 was considered statistically significant.

Results

Glass ionomer-based FS showed the highest PM in all three surface conditions (p values < 0.05 were considered statistically significant). No significant difference in PM was observed between resin-based FS and amorphous calcium phosphate-containing FS (p > 0.05). Resin-based FS showed significantly greater SBS in all three surface conditions compared to glass ionomer-based FS. SEM observations showed that saliva contamination led to gaps at the enamel–sealant interface.

Conclusion

Neither cotton pellet-drying nor air-drying effectively removed saliva from the contaminated enamel surface. Glass ionomer-based FS showed the highest PM and the lowest SBS in contaminated and noncontaminated conditions. The highest SBS was obtained with resin-based FS.

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Data availability

The datasets analyzed during the current study are available from the corresponding author on reasonable request.

Code availability

Not applicable.

Abbreviations

ACP:

Amorphous calcium phosphate

Bis-GMA:

Bisphenol A-glycidyl methacrylate

FS:

Fissure sealant

MPa:

Megapascals

PM:

Proportion of microleakage

SBS:

Shear bond strength

SD:

Standard deviation

SEM:

Scanning electron microscopy

UDMA:

Urethane dimethacrylate

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Acknowledgements

The authors thank the Vice-Chancellery of Research of Shiraz University of Medical Sciences, for supporting this research (Grant No. 96-15804, 97-18231). The authors also thank Dr. M. Vossoughi of the Center for Improvement, Shiraz Dental School, for the statistical analysis, and K. Shashok (AuthorAID in the Eastern Mediterranean) for help with the English in the manuscript. This manuscript reports research done in partial fulfillment of the requirements for the PhD degree awarded to one of the authors, Dr. Tayebeh Doroudizadeh.

Funding

Funding for this study was provided by vice-chancellery of Shiraz University of Medical Sciences for the design of the study, collection, analysis, and interpretation of data.

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Authors and Affiliations

  1. Oral and Dental Disease Research Center, Department of Pediatric Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran

    Mahtab Memarpour & Azade Rafiee

  2. Oral and Dental Disease Research Center, Department of Operative Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran

    Fereshteh Shafiei

  3. Student Research Committee, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran

    Tayebeh Dorudizadeh & Sahba Kamran

Authors
  1. Mahtab Memarpour
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  2. Azade Rafiee
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  3. Fereshteh Shafiei
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  4. Tayebeh Dorudizadeh
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  5. Sahba Kamran
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Contributions

Dr. Memarpour: conceptualized and designed the study, supervised data collection, critically reviewed the manuscript, and approved the final manuscript as submitted. Dr. Rafiee: conceptualized and designed the study, collected and interpreted the data, drafted the manuscript, and approved the final manuscript as submitted. Dr. Fereshteh Shafiei, Dr. Tayebeh Dorudizadeh, and Dr. Sahba Kamran: conceptualized the study, participated in data acquisition and analysis, revised the manuscript critically for important intellectual content, and approved the final manuscript as submitted. All authors approved the final manuscript as submitted and agree to be accountable for all aspects of the work.

Corresponding author

Correspondence to Azade Rafiee.

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The authors declare that there is no conflict of interest regarding the publication of this paper.

Ethics approval and consent to participate

The study was approved by the Ethics Review Committee of the School of Dentistry, Shiraz University of Medical Sciences (IR.SUMS.REC.1397.924).

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Not applicable.

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Memarpour, M., Rafiee, A., Shafiei, F. et al. Adhesion of three types of fissure sealant in saliva-contaminated and noncontaminated conditions: an in vitro study. Eur Arch Paediatr Dent 22, 813–821 (2021). https://doi.org/10.1007/s40368-021-00626-1

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  • DOI: https://doi.org/10.1007/s40368-021-00626-1

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