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Toxic Effects of Tetrabromobisphenol A on Human Hepatocellular Carcinoma Cells(HepG2) Evaluated Using RNA-Seq

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Abstract

Tetrabromobisphenol A(TBBPA) is used as a brominated flame retardant and can easily release into environment. It has been detected in a variety of environmental media and even in food, making it a new pollutant that is potentially harmful to environment and human health. However, the toxicity and underlying mechanism of TBBPA remain to be explored. TBBPA is actively metabolized in the liver, so it is vital to study the hepatocellular toxicity of TBBPA. In this study, we treated hepatocellular carcinoma cells(HepG2) with TBBPA and searched the differentially expressed genes(DEGs) triggered by TBBPA exposure. RNA-seq analysis showed that the expression of genes, such as CLIC1 and GATSL1, were significantly down-regulated and PTENP1 was significantly up-regulated after TBBPA exposure, and these genes were involved in cell proliferation and metabolism. The down-regulated genes were enriched to peroxisome proliferator-activated receptor(PPAR) signaling pathway and up-regulated genes were enriched to Janus Kinase-signal transduces and activators of transcription(JAK-STAT) signaling pathway, which play a role in regulating cell growth. Overall, our transcriptome analysis obtained for TBBPA-treated HepG2 cells implies that TBBPA affects the cell proliferation and growth.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos.22234008, 21927807, 22021003).

We thank Dr. ZENG Jianming(University of Macau, China), all the members of his bioinformatics team and biotrainees, for generously sharing their experience and codes.

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Authors and Affiliations

  1. The State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 10085, P. R. China

    Hailin Wang

  2. Sino-Danish College, University of the Chinese Academy of Sciences, Beijing, 100049, P. R. China

    Haoran Chang & Hailin Wang

  3. Hangzhou Institute for Advanced Study, University of the Chinese Academy of Sciences, Hangzhou, 310024, P. R. China

    Qingjiang Ding & Hailin Wang

  4. University of the Chinese Academy of Sciences, Beijing, 100049, P. R. China

    Haoran Chang, Qingjiang Ding & Hailin Wang

Authors
  1. Haoran Chang
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  2. Qingjiang Ding
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  3. Hailin Wang
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Correspondence to Hailin Wang.

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The authors declare no conflicts of interest.

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Chang, H., Ding, Q. & Wang, H. Toxic Effects of Tetrabromobisphenol A on Human Hepatocellular Carcinoma Cells(HepG2) Evaluated Using RNA-Seq. Chem. Res. Chin. Univ. 39, 395–398 (2023). https://doi.org/10.1007/s40242-023-3069-2

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  • DOI: https://doi.org/10.1007/s40242-023-3069-2

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