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Electrochemical immunoassay of E. coli in urban sludge using electron mediator-mediated enzymatic catalysis and gold nanoparticles for signal amplification

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Abstract

An electrochemical immunosensor was developed for sensitive assay of E. coli in urban sludge, in which electron mediator-mediated enzymatic catalysis and gold nanoparticles(AuNPs) were utilized for signal amplification. The immnuosensing platform chitosan-thionine(chit-thio)/poly(amidoamine) dendrimer-encapsulated AuNPs [PAMAM(Au)] composites were first prepared from chit-thio and PAMAM(Au) using the layer-by-layer method to provide a matrix for high-stability and high-bioactivity bindings of the capture antibody(cAb). Moreover, the {dAb-AuNPs-HRP} nanoprobes were designed to exploit the amplification effect of the carrier AuNPs due to the loading amounts of horseradish peroxidase(HRP) and the detection antibody(dAb). The sandwich-type immunoassay was then successfully used to assay E. coli based on the oxidation of thionine as a result of H2O2-induced enzymatic catalytic reaction by HRP. This study presents a powerful tool in electrochemical immunoassay for E. coli detection with rapid response, high-sensitivity and high-specificity, providing a potential new tool for feasibility assessment of sludge recycle.

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Authors and Affiliations

  1. School of Energy and Power Engineering, Jiangsu University, Zhenjiang, 212013, P. R. China

    Wenjie Lu, Rongjin Xu, Jianzhong Shen & Changfeng Li

  2. School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, P. R. China

    Xinai Zhang

Authors
  1. Wenjie Lu
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  2. Rongjin Xu
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  3. Xinai Zhang
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  4. Jianzhong Shen
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  5. Changfeng Li
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Corresponding authors

Correspondence to Xinai Zhang or Changfeng Li.

Additional information

Supported by the National Natural Science Foundation of China(Nos.21205051, 51608235) and the Key Project of the Ministry of Education of China(No.210078).

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Lu, W., Xu, R., Zhang, X. et al. Electrochemical immunoassay of E. coli in urban sludge using electron mediator-mediated enzymatic catalysis and gold nanoparticles for signal amplification. Chem. Res. Chin. Univ. 34, 101–106 (2018). https://doi.org/10.1007/s40242-017-7254-z

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  • DOI: https://doi.org/10.1007/s40242-017-7254-z

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