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Electrospun PCL scaffold modified with chitosan nanoparticles for enhanced bone regeneration

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Abstract

The encapsulation of ascorbic acid within chitosan nanoparticles (CHNs), embedded in a fibrous structure of a dexamethasone (Dex)-loaded PCL scaffold, provides a new plan for osteogenic differentiation of mesenchymal stem cells. This electrospun PCL fibrous scaffold can release Dex, as bone differentiation initiator, and ascorbic acid, as bone differentiation enhancer, in an approximately sustained release pattern for about 2 weeks. Ascorbic acid-loaded CHNs were prepared by electrospraying a mixture of chitosan and ascorbic acid, and Dex-containing PCL fibers were prepared by electrospinning a mixture of PCL and Dex. The final PCL/chitosan bilayer scaffolds were obtained by the sequential employment of electrospinning and electrospraying methods. Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) confirmed that the CHNs were successfully incorporated into the fibrous PCL matrix. The improved proliferation of hMSCs cultured on the PCL/chitosan scaffolds was also verified. Osteogenic assays showed an increase in alkaline phosphatase activity and mineral deposits. The expression of bone-specific genes also confirmed the osteogenic differentiation of cells cultured on these PCL/chitosan bilayer scaffolds. Dual-drug-loaded PCL/chitosan scaffold enhanced the osteoblast differentiation of hMSC cells and can be served as a potential scaffold for bone tissue engineering.

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Acknowledgements

The authors declare that no funding has been received for the conduct of this study. The authors would like to thank Tarbiat Modares University and Royan Institute for Stem Cell Biology and Technology for their technical maintenance.

Funding

This study was funded by Tarbiat Modares University and Royan Institute for Stem Cell Biology and Technology.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran

    Ameneh Seddighian & Fariba Ganji

  2. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran

    Mohamadreza Baghaban-Eslaminejad

  3. Department of Biotechnology, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran

    Fatemeh Bagheri

Authors
  1. Ameneh Seddighian
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  2. Fariba Ganji
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  3. Mohamadreza Baghaban-Eslaminejad
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  4. Fatemeh Bagheri
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Correspondence to Fariba Ganji or Mohamadreza Baghaban-Eslaminejad.

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Ameneh Seddighian declares that she has no conflict of interest. Fariba Ganji declares that she has no conflict of interest. Mohamadreza Baghaban-Eslaminejad declares that she has no conflict of interest. Fatemeh Bagheri declares that she has no conflict of interest.

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Seddighian, A., Ganji, F., Baghaban-Eslaminejad, M. et al. Electrospun PCL scaffold modified with chitosan nanoparticles for enhanced bone regeneration. Prog Biomater 10, 65–76 (2021). https://doi.org/10.1007/s40204-021-00153-8

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  • DOI: https://doi.org/10.1007/s40204-021-00153-8

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