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Elimination and detoxification of phenanthrene assisted by a laccase from halophile Alkalibacillus almallahensis

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Abstract

Phenanthrene (Phe), a tricyclic Polycyclic Aromatic Hydrocarbon (PAH), is found in high concentrations as a pollutant in various environments. In this study, the removal or (oxidizing) ability of Phe by a laccase from Alkalibacillus almallahensis was investigated. The laccase (12 U mL−1) was able to remove 63% of Phe (50 mg L−1) under optimal conditions of 40 °C, pH 8, 1.5 M NaCl and in the presence of 1 mM HBT as a laccase mediator after a 72 h incubation period. The results for the effect of different solvents, ionic and non-ionic surfactants on the activity of the halophilic laccase towards Phe showed that the addition of these compounds increase removal efficiency and complete enzymatic removal of Phe will achieve in a solution of 5% (v/v) acetone and 1.5% tween 80. The kinetic parameters Km and Vmax of laccase-catalyzed removal of the substrate were determined as 0.544 mM and 0.882 µmol h−1 mg−1, respectively. A microtoxicity study with respect to the inhibition of algal growth showed a decrease in toxicity of the laccase-treated Phe solution.

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The data supporting the results of this study will be made available by the corresponding author, upon reasonable request.

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Authors and Affiliations

  1. School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, 14176, Iran

    Shiler Valizadeh, Sonia Mohamadnia, Elaheh Rahimi & Omid Tavakoli

  2. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155–6451, 1417614411, Tehran, Iran

    Shahla Rezaei & Mohammad Ali Faramarzi

Authors
  1. Shiler Valizadeh
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  2. Shahla Rezaei
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  3. Sonia Mohamadnia
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  4. Elaheh Rahimi
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  5. Omid Tavakoli
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  6. Mohammad Ali Faramarzi
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Contributions

Shiler Valizadeh, Shahla Rezaei, and Elaheh Rahimi designed the study and experiments. Omid Tavakoli and Mohammad Ali Faramarzi instructed the experiments. Shiler Valizadeh, Shahla Rezaei, Sonia Mohamadnia, and Elaheh Rahimi performed the experiments and data analysis. Shiler Valizadeh, Shahla Rezaei, and Sonia Mohamadnia drafted the manuscript. Omid Tavakoli and Mohammad Ali Faramarzi writing-reviewing and editing, the manuscript. All authors read and approved the final manuscript.

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Correspondence to Omid Tavakoli or Mohammad Ali Faramarzi.

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Valizadeh, S., Rezaei, S., Mohamadnia, S. et al. Elimination and detoxification of phenanthrene assisted by a laccase from halophile Alkalibacillus almallahensis. J Environ Health Sci Engineer 20, 227–239 (2022). https://doi.org/10.1007/s40201-021-00771-1

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