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ACE I/D gene polymorphisms and polycystic ovary syndrome manifestations

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Abstract

Objective

Polycystic ovary syndrome (PCOS) is a common multifactorial endocrine disorder affecting women of reproductive age. ACE enzyme is involved in the physiopathology of the ovarian system, and there are inconsistencies between studies regarding the association between ACE gene variants and PCOS. The objective of this study is to evaluate the association between ACE I/D gene polymorphisms and PCOS, as well as its clinical manifestations, in Iranian women with PCOS.

Design

This study included 140 patients with PCOS and 153 controls without the disease. Samples were collected from Yas Hospital Complex in Tehran–Iran during 2018 to 2022. Genomic DNA was obtained from whole blood samples using salt extraction, and genotyping was carried out using polymerase chain reaction (PCR).

Results

Variants of DD, ID, and II were observed in 31.4, 44.3, and 24.3% of PCOS, and 38.6, 44.1, and 17.2% of control group, respectively. The frequency of ACE gene variants did not differ between PCOS patients and control group. A significant difference was observed between the frequency of elevated LH to FSH ratio > 2 and ACE gene polymorphisms in patients with PCOS (OR: 0.32 (0.12–0.88), P value 0.024) with lower frequency observed in D allele carriers.

Conclusion

This study indicate that although ACE I/D variants frequency in PCOS women is similar to non-PCOS women, it may be involved in the pathogenesis of the disease through mechanisms regulating steroidogenesis in the ovary and suggests that ACE might be related to exacerbated clinical manifestations of PCOS which requires further investigations.

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Author notes

  1. Mojgan Asadi and Zeynab Nickhah Klashami are co-first authors and contributed equally to this work.

Authors and Affiliations

  1. Diabetes Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Mojgan Asadi

  2. Metabolomics and Genomics Research Center, Endocrinology and Metabolism Molecular- Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Mojgan Asadi

  3. Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Zeynab Nickhah Klashami, Nekoo Panahi, Yodit Oheb & Mahsa M. Amoli

  4. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Nekoo Panahi & Sara Khorasani

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  1. Mojgan Asadi
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Correspondence to Nekoo Panahi or Mahsa M. Amoli.

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All procedures performed in studies involving human participants were in accordance with the ethics protocol of the institutional and/or national Research Committee and the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This study was approved by Endocrine and Metabolic Research Institute (EMRI) Ethics Committee (IR.TUMS.EMRI.REC.1400.047).

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Asadi, M., Nickhah Klashami, Z., Panahi, N. et al. ACE I/D gene polymorphisms and polycystic ovary syndrome manifestations. J Diabetes Metab Disord (2023). https://doi.org/10.1007/s40200-023-01335-w

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