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Association analysis between the VDR gene variants and type 2 diabetes

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Abstract

Background

Type 2 diabetes mellitus (T2DM) is recognized as a complex metabolic which has affected the lives of millions of people around the world. Vitamin D receptor (VDR) gene polymorphisms have been suggested to be a vital contributor to the development of T2DM. However, the association between VDR gene polymorphisms and T2DM remains controversial. We have investigated the association between two VDR gene polymorphisms (rs731236 and rs1544410) and T2DM in an Iranian population.

Methods

A total of 148 T2DM patients and 100 normal controls were recruited in this study. We used polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis to perform genotyping.

Results

The results of the present research revealed that the frequency of the rs731236 C allele was significantly higher in T2DM patients than in normal controls (p = 0.044). The CC genotype of rs731236 was connected with an increased risk of T2DM (OR = 2.85, 95% CI = 1.06–7.69, p = 0.039). However, no significant difference in the frequency of the rs1544410 C allele between T2DM patients and normal controls was observed (p = 0.918).

Conclusion

Our findings were suggestive of the rs731236 polymorphism of the VDR as a risk factor for developing T2DM in the Iranian population, while rs1544410 polymorphism may not be associated with T2DM susceptibility. Further research is needed to approve these findings in other populations and to clarify the underlying mechanisms involved in such an association.

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Abbreviations

T2DM:

type 2 diabetes mellitus International Diabetes Federation (IDF)

VDR:

Vitamin D receptor

SNPs:

single nucleotide polymorphisms

HbA1c:

hemoglobin A1c

FBS:

fasting blood sugar

BMI:

body mass index

PCR:

polymerase chain reaction

SD:

standard deviation

OR:

odds ratios

CI:

confidence intervals

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Acknowledgements

The authors thank to T2DM patients who participated in this study.

Funding

No funding was obtained.

Author information

Author notes

  1. Shabnam Salehizadeh and Sara Ramezani contributed equally to co-first authorship.

  2. Mojgan Asadi and Mandana Hasanzad contributed equally to co-corresponding authorship.

Authors and Affiliations

  1. Medical Genomics Research Center, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

    Shabnam Salehizadeh, Sara Ramezani, Seyed Hamid Jamaldini & Farhad Adhami Moghadam

  2. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Mojgan Asadi

  3. Department of Community Medicine, Zabol University of Medical Sciences, Zabol, Iran

    Mahdi Afshari

  4. Personalized Medicine Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Mandana Hasanzad

Authors
  1. Shabnam Salehizadeh
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Contributions

SHS and SR: Investigation, Methodology; MA: Statistical Analysis; MA, FAM and SHJ; Resources; and MH Conceptualization, Validation. All authors contributed to and approved the final version of the manuscript.

Corresponding authors

Correspondence to Mojgan Asadi or Mandana Hasanzad.

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Competing of interest

There are no conflicts of interest declared by the authors.

Consent for publication

The present study research is approved by endocrinology and metabolism clinical institute ethics committee (IR.IAU.PS.REC.1399.032; IR.IAU.PS.REC.1399.031) and all T2DM patients signed written consent forms.

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Salehizadeh, S., Ramezani, S., Asadi, M. et al. Association analysis between the VDR gene variants and type 2 diabetes. J Diabetes Metab Disord (2023). https://doi.org/10.1007/s40200-023-01323-0

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