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Therapeutic effects of in vivo-differentiated stem cell and Matricaria chamomilla L. Oil in diabetic rabbit

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Journal of Diabetes & Metabolic Disorders Aims and scope Submit manuscript

Abstract

Background

The main goal of diabetes therapy is to control blood glucose levels.

Objectives

In this study, the effect of Matricaria chamomilla L. oil as an herbal agent, on therapeutic properties of poly L-lactic acid-based (PLLA) scaffold loaded with differentiated stem cells, is examined in the diabetic rabbit.

Methods

Adipose mesenchymal stem cells (AMSCs) were isolated from male New Zealand White rabbits and after seeding on the PLLA scaffold differentiated in the pancreatic region. In vivo differentiation of AMSCs toward pancreatic progenitor cells was evaluated by quantitative analysis of gene expressions and immunohistochemistry. Then, one normal and five diabetic groups including blank diabetic, scaffold, oil + scaffold, and differentiated cell + scaffold or oil + scaffold were assessed after 21 days of treatment. After the assessment, the diabetic groups were evaluated by clinical parameters and pancreatic histological sections.

Results

It was found that AMSCs were differentiated to insulin-producing cells (IPCs) in the pancreatic environment which then used for implantation. Blood glucose in the oil + scaffold, cell + scaffold, and oil + cell + scaffold groups showed a significant decrease after 21 days. In the above mentioned three groups, insulin secretion was increased significantly. Chamomile oil also caused a significant decrease in High-density lipoprotein (HDL), Low-density lipoprotein (LDL), and total cholesterol levels. According to histological sections results, in cell + scaffold and oil + cell + scaffold groups, β cells were significantly increased compared to blank diabetic group.

Conclusions

Together these data demonstrated chamomile oil along with in vivo-differentiated stem cell is a promising new treatment for diabetes.

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Abbreviations

AMSCs:

Adipose mesenchymal stem cells

MSCs:

Mesenchymal stem cells

PLLA:

Poly L-lactic acid

IPCs:

Insulin-producing cells

HDL:

High-density lipoprotein

LDL:

Low-density lipoprotein

STZ:

Streptozotocin

FTIR:

Fourier transform infrared

SEM:

Scanning electron microscopy

ROS:

Reactive oxygen species

H&E:

Hematoxylin and Eosin

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Authors and Affiliations

  1. Department of Anatomy, School of Veterinary Medicine, Shiraz University, P.O.Box: 14115-111, Fars, Iran

    Afsaneh Fazili & Soghra Gholami

  2. Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran

    Mojgan Sheikhpour

  3. Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran

    Mojgan Sheikhpour

  4. North Tehran Branch, Islamic Azad Universities, Tehran, Iran

    Pegah Pousti

Authors
  1. Afsaneh Fazili
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  2. Soghra Gholami
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  3. Mojgan Sheikhpour
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  4. Pegah Pousti
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Correspondence to Soghra Gholami.

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Fazili, A., Gholami, S., Sheikhpour, M. et al. Therapeutic effects of in vivo-differentiated stem cell and Matricaria chamomilla L. Oil in diabetic rabbit. J Diabetes Metab Disord 19, 453–460 (2020). https://doi.org/10.1007/s40200-020-00530-3

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