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Antifouling Activities of Antagonistic Marine Bacterium Pseudomonas putida Associated with an Octopus

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Bacterial symbionts associated with an octopus were isolated and their intra and extracellular extracts were tested for the antagonistic activities against biofilm-forming bacteria. Among the three antagonistic bacterial symbionts, both intra and extracellular extracts of the bacterium, Pseudomonas putida exhibited strong antibacterial activity against biofilm bacteria. Results also showed that both intra and extracellular extracts of P. putida significantly reduced the biofilm formation on test surfaces. The antifouling coating developed with bacterial extracts of P. putida significantly reduced the recruitment of biofouling organisms over a period of 50 days in the coastal waters. The bioactive fractions were partially purified using chromatographic methods such as thin-layer chromatography and high performance liquid chromatography. Based on the findings, it is concluded that potential antifouling compounds could be isolated from the antagonistic bacteria isolated from the octopus.

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Acknowledgments

The authors are thankful to University Grants Commission for providing financial support through the major project scheme (Ref. F. No: 39-294/2010 SR).

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Authors and Affiliations

  1. Centre for Marine Science and Technology, Manonmanium Sundaranar University, Rajakkamangalam, Kanyakumari, Tamilnadu, India

    N. Viju & S. Mary Josephine Punitha

  2. Department of Marine Biology, Faculty of Marine Sciences, King Abdulaziz University, Jeddah, Saudi Arabia

    S. Satheesh

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  1. N. Viju
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Viju, N., Satheesh, S. & Punitha, S.M.J. Antifouling Activities of Antagonistic Marine Bacterium Pseudomonas putida Associated with an Octopus. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 87, 1113–1124 (2017). https://doi.org/10.1007/s40011-015-0691-x

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  • DOI: https://doi.org/10.1007/s40011-015-0691-x

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