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Evaluation and application of cryosectioning in undecalcified hard tissues in cartilage and bone regenerative medicine

  • Review Article
  • Regenerative Medicine
  • Published:
Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

This article overall describes the development of histological method especially, cartilage and bone research in regenerative medicine. Kawamoto’s film method, which has been recently introduced into our laboratory, has been applied in various fields that make sectioning of hard tissues easier than the conventional method. Moreover, this method also does not require the time-consuming chemical fixation and/or decalcification process. Kawamoto method involves the use of the adhesive plastic film instead of a cover glass where the thin tissue sections are attached efficiently at low temperatures (-25°C). Furthermore, the histological method preserves the enzymatic activity in the fresh sections in comparison to that of chemically-treated tissue sections. In fact, we used this Kawamoto’s film method in one of our researches in which drug repositioning was employed for cartilage regeneration. Some of the Kawamoto-processed tissue sections are featured in this review article. Therefore, the application of this tissue preparation technique allowed effective and histological and histochemical studies within a shorter preparation time with ease and convenience. In the future, this Kawamoto method may offer further applications in the preparation of a more diverse tissues and samples not only in the preclinical but also in the clinical studies.

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Authors and Affiliations

  1. Department of Anatomy, School of Medicine, Konkuk University, Seoul, Korea

    Seung Hwa Park

  2. IT Design Fusion Program, Graduate School Of NID Fusion Technology, Seoul National University of Science and Technology, Seoul, Korea

    Jeewon Yoon

  3. Department of Veterinary Medicine, College of Veterinary Medicine, Konkuk University, Seoul, Korea

    Jae Hyun Park, Bo Young Kim, Judee Grace E. Nemeno, Ernesto C. Balolong Jr., Yuna Han, Kyung Mi Lee, Soojung Lee & Jeong Ik Lee

  4. Regenerative Medicine Laboratory, Center for Stem Cell Research, Department of Biomedical Science and Technology, Institute of Biomedical Science & Technology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 143-701, Korea

    Jeong Ik Lee

  5. Regeniks Co., Ltd., Seoul, Korea

    Jeong Ik Lee

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  1. Seung Hwa Park
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  2. Jeewon Yoon
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Correspondence to Soojung Lee or Jeong Ik Lee.

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Park, S.H., Yoon, J., Park, J.H. et al. Evaluation and application of cryosectioning in undecalcified hard tissues in cartilage and bone regenerative medicine. Tissue Eng Regen Med 12 (Suppl 2), 94–104 (2015). https://doi.org/10.1007/s13770-015-0433-4

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  • DOI: https://doi.org/10.1007/s13770-015-0433-4

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