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Recent Advances in Transbronchial Lung Cryobiopsy

  • Interventional Pulmonology (Sixto Alejandro Arias, Section Editor)
  • Published:
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Abstract

Purpose of Review

This article will discuss new advances in cryoprobe technology and procedural utilization for the diagnosis of lung cancer and parenchymal lung diseases.

Recent Findings

Newly developed smaller cryoprobes can offer similar diagnostic advantages of standard cryoprobes when used as a diagnostic modality in a variety of lung conditions.

Summary

Bronchoscopic lung cryobiopsy is an innovative diagnostic technique for intraparenchymal lung diseases. Over the past decade, research has been dedicated to evaluating the safety profile of this technique, developing a standard procedural approach, and exploring the utility of this approach for a variety of indications. In this article, we will review recent developments for the diagnostic utility of cryobiopsy in interstitial lung diseases, lung cancer, and lung transplant. We will also describe a technical approach for the procedure and evaluate complications and areas of ongoing research. With a similar safety profile and superior diagnostic yield compared to transbronchial forceps biopsy, ongoing research and development may pave the way for transbronchial lung cryobiopsy to become a first-line, minimally invasive diagnostic approach for many intraparenchymal lung diseases.

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References

  1. Raghu G, Remy-Jardin M, Myers JL, Richeldi L, Ryerson CJ, Lederer DJ, Behr J, Cottin V, Danoff SK, Morell F, Flaherty KR, Wells A, Martinez FJ, Azuma A, Bice TJ, Bouros D, Brown KK, Collard HR, Duggal A, Galvin L, Inoue Y, Jenkins RG, Johkoh T, Kazerooni EA, Kitaichi M, Knight SL, Mansour G, Nicholson AG, Pipavath SNJ, et al. Diagnosis of idiopathic pulmonary fibrosis. an official ATS/ERS/JRS/ALAT clinical practice guideline. Am J Respir Crit Care Med. 2018;198:e44–e68.

  2. Raghu G, Remy-Jardin M, Ryerson CJ, Myers JL, Kreuter M, Vasakova M, Bargagli E, Chung JH, Collins BF, Bendstrup E, Chami HA, Chua AT, Corte TJ, Dalphin J-C, Danoff SK, Diaz-Mendoza J, Duggal A, Egashira R, Ewing T, Gulati M, Inoue Y, Jenkins AR, Johannson KA, Johkoh T, Tamae-Kakazu M, Kitaichi M, Knight SL, Koschel D, Lederer DJ, et al. Diagnosis of hypersensitivity pneumonitis in adults: an official ATS/JRS/ALAT clinical practice guideline. Am J Respir Crit Care Med. 2020;202:e36–69.

    PubMed  PubMed Central  Google Scholar 

  3. Kaarteenaho R. The current position of surgical lung biopsy in the diagnosis of idiopathic pulmonary fibrosis. Respir Res. 2013;14:43.

    PubMed  PubMed Central  Google Scholar 

  4. Utz JP, Ryu JH, Douglas WW, Hartman TE, Tazelaar HD, Myers JL, Allen MS, Schroeder DR. High short-term mortality following lung biopsy for usual interstitial pneumonia. Eur Respir J. 2001;17:175–9.

    CAS  PubMed  Google Scholar 

  5. Nasu S, Okamoto N, Suzuki H, Shiroyama T, Tanaka A, Samejima Y, Kanai T, Noda Y, Morita S, Morishita N, Ueda K, Kawahara K, Hirashima T. Comparison of the utilities of cryobiopsy and forceps biopsy for peripheral lung cancer. Anticancer Res. 2019;39:5683–8.

    PubMed  Google Scholar 

  6. Koslow M, Edell ES, Midthun DE, Mullon JJ, Kern RM, Nelson DR, Sakata KK, Moua T, Roden AC, Yi ES, Reisenauer JS, Decker PA, Ryu JH. Bronchoscopic cryobiopsy and forceps biopsy for the diagnostic evaluation of diffuse parenchymal lung disease in clinical practice. Mayo Clin Proc Innov Qual Outcomes. 2020;4:565–74.

    PubMed  PubMed Central  Google Scholar 

  7. Schumann C, Hetzel J, Babiak AJ, Merk T, Wibmer T, Möller P, Lepper PM, Hetzel M. Cryoprobe biopsy increases the diagnostic yield in endobronchial tumor lesions. J Thorac Cardiovasc Surg. 2010;140:417–21.

    PubMed  Google Scholar 

  8. Pourabdollah M, Shamaei M, Karimi S, Karimi M, Kiani A, Jabbari HR. Transbronchial lung biopsy: the pathologist’s point of view. Clin Respir J. 2016;10:211–6.

    PubMed  Google Scholar 

  9. Huang Y, Huang H, Li Q, Browning RF, Parrish S, Turner JF, Zarogoulidis K, Kougioumtzi I, Dryllis G, Kioumis I, Pitsiou G, Papaiwannou A, Lampaki S, Machairiotis N, Katsikogiannis N, Madesis A, Karaiskos T, Li Z, Zarogoulidis P. Transbronchial lung biopsy and pneumothorax. J Thorac Dis. 2014;6:S443–7.

    PubMed  PubMed Central  Google Scholar 

  10. Arya R, Boujaoude Z, Rafferty WJ, Abouzgheib W. Usefulness and safety of transbronchial biopsy with large forceps during flexible bronchoscopy. Proc (Bayl Univ Med Cent). 2020;34:232–6.

    Google Scholar 

  11. Babiak A, Hetzel J, Krishna G, Fritz P, Moeller P, Balli T, Hetzel M. Transbronchial cryobiopsy: a new tool for lung biopsies. Respiration. 2009;78:203–8.

    PubMed  Google Scholar 

  12. Kho SS, Chan SK, Yong MC, Tie ST. Performance of transbronchial cryobiopsy in eccentrically and adjacently orientated radial endobronchial ultrasound lesions. ERJ Open Research. 2019;5.

  13. Mehrad M, Colby TV, Rossi G, Cavazza A. Transbronchial cryobiopsy in the diagnosis of fibrotic interstitial lung disease. Arch Pathol Lab Med. 2020;144:1501–8.

    PubMed  Google Scholar 

  14. Yarmus LB, Semaan RW, Arias SA, Feller-Kopman D, Ortiz R, Bösmüller H, Illei PB, Frimpong BO, Oakjones-Burgess K, Lee HJ. A randomized controlled trial of a novel sheath cryoprobe for bronchoscopic lung biopsy in a porcine model. Chest. 2016;150:329–36.

    PubMed  Google Scholar 

  15. Udagawa H, Kirita K, Naito T, Nomura S, Ishibashi M, Matsuzawa R, Hisakane K, Usui Y, Matsumoto S, Yoh K, Niho S, Ishii G, Goto K. Feasibility and utility of transbronchial cryobiopsy in precision medicine for lung cancer: prospective single-arm study. Cancer Sci. 2020;111:2488–98.

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Hetzel J, Linzenbold W, Boesmueller H, Enderle M, Poletti V. Evaluation of Efficacy of a new cryoprobe for transbronchial cryobiopsy: a randomized, controlled in vivo animal study. Respiration. 2020;99:248–56.

    PubMed  Google Scholar 

  17. Ganganah O, Guo SL, Chiniah M, Li YS. Efficacy and safety of cryobiopsy versus forceps biopsy for interstitial lung diseases and lung tumours: a systematic review and meta-analysis. Respirology. 2016;21:834–41.

    PubMed  Google Scholar 

  18. Horbaniuc BD. Refrigeration and Air-Conditioning. Encyclopedia of Energy. Elsevier; 2004. p. 261–89. https://www.sciencedirect.com/topics/earth-and-planetary-sciences/joule-thomson-effect.

  19. Maldonado F, Danoff SK, Wells AU, Colby TV, Ryu JH, Liberman M, Wahidi MM, Frazer L, Hetzel J, Rickman OB, Herth FJF, Poletti V, Yarmus LB. Transbronchial cryobiopsy for the diagnosis of interstitial lung diseases: CHEST guideline and expert panel report. Chest. 2020;157:1030–42.

    PubMed  Google Scholar 

  20. Ravaglia C, Wells AU, Tomassetti S, Gurioli C, Gurioli C, Dubini A, Cavazza A, Colby TV, Piciucchi S, Puglisi S, Bosi M, Poletti V. Diagnostic yield and risk/benefit analysis of trans-bronchial lung cryobiopsy in diffuse parenchymal lung diseases: a large cohort of 699 patients. BMC Pulm Med. 2019;19:16.

    PubMed  PubMed Central  Google Scholar 

  21. Troy LK, Grainge C, Corte TJ, Williamson JP, Vallely MP, Cooper WA, Mahar A, Myers JL, Lai S, Mulyadi E, Torzillo PJ, Phillips MJ, Jo HE, Webster SE, Lin QT, Rhodes JE, Salamonsen M, Wrobel JP, Harris B, Don G, Wu PJC, Ng BJ, Oldmeadow C, Raghu G, Lau EMT. Cryobiopsy versus open lung biopsy in the diagnosis of interstitial lung disease alliance (COLDICE) investigators. Diagnostic accuracy of transbronchial lung cryobiopsy for interstitial lung disease diagnosis (COLDICE): a prospective, comparative study. Lancet Respir Med. 2020;8:171–181.

  22. Lentz RJ, Argento AC, Colby TV, Rickman OB, Maldonado F. Transbronchial cryobiopsy for diffuse parenchymal lung disease: a state-of-the-art review of procedural techniques, current evidence, and future challenges. J Thorac Dis. 2017;9:2186–203.

    PubMed  PubMed Central  Google Scholar 

  23. DiBardino DM, Lanfranco AR, Haas AR. Bronchoscopic cryotherapy. clinical applications of the cryoprobe, cryospray, and cryoadhesion. Ann American Thorac Soc. 2016;13:1405–1415.

  24. Pajares V, Puzo C, Castillo D, Lerma E, Montero MA, Ramos-Barbón D, Amor-Carro O, Gil de Bernabé A, Franquet T, Plaza V, Hetzel J, Sanchis J, Torrego A. Diagnostic yield of transbronchial cryobiopsy in interstitial lung disease: a randomized trial. Respirology. 2014;19:900–906.

  25. Tomassetti S, Ravaglia C, Wells AU, Cavazza A, Colby TV, Rossi G, Ley B, Ryu JH, Puglisi S, Arcadu A, Marchi M, Sultani F, Martinello S, Donati L, Gurioli C, Gurioli C, Tantalocco P, Hetzel J, Dubini A, Piciucchi S, Klersy C, Lavorini F, Poletti V. Prognostic value of transbronchial lung cryobiopsy for the multidisciplinary diagnosis of idiopathic pulmonary fibrosis: a retrospective validation study. Lancet Respir Med. 2020;8:786–94.

    PubMed  Google Scholar 

  26. Dhooria S, Sehgal IS, Aggarwal AN, Behera D, Agarwal R. Diagnostic yield and safety of cryoprobe transbronchial lung biopsy in diffuse parenchymal lung diseases: systematic review and meta-analysis. Respir Care. 2016;61:700–12.

    PubMed  Google Scholar 

  27. Iftikhar IH, Alghothani L, Sardi A, Berkowitz D, Musani AI. Transbronchial lung cryobiopsy and video-assisted thoracoscopic lung biopsy in the diagnosis of diffuse parenchymal lung disease. a meta-analysis of diagnostic test accuracy. Ann Am Thorac Soc. 2017;14:1197–1211.

  28. Unterman A, Wand O, Fridel L, Edelstein E, Pertzov B, Kramer MR. High diagnostic accuracy of transbronchial cryobiopsy in fibrotic interstitial lung diseases compared to final explant diagnosis. Respiration. 2019;98:421–7.

    PubMed  Google Scholar 

  29. Hetzel J, Eberhardt R, Herth FJF, Petermann C, Reichle G, Freitag L, Dobbertin I, Franke KJ, Stanzel F, Beyer T, Möller P, Fritz P, Ott G, Schnabel PA, Kastendieck H, Lang W, Morresi-Hauf AT, Szyrach MN, Muche R, Shah PL, Babiak A, Hetzel M. Cryobiopsy increases the diagnostic yield of endobronchial biopsy: a multicentre trial. Eur Respir J. 2012;39:685–90.

    CAS  PubMed  Google Scholar 

  30. Hetzel J, Eberhardt R, Petermann C, Gesierich W, Darwiche K, Hagmeyer L, Muche R, Kreuter M, Lewis R, Ehab A, Boeckeler M, Haentschel M. Bleeding risk of transbronchial cryobiopsy compared to transbronchial forceps biopsy in interstitial lung disease - a prospective, randomized, multicentre cross-over trial. Respir Res. 2019;20:140.

    PubMed  PubMed Central  Google Scholar 

  31. Pajares V, Núñez-Delgado M, Bonet G, Pérez-Pallarés J, Martínez R, Cubero N, Zabala T, Cordovilla R, Flandes J, Disdier C, Torrego A. MULTICRIO Group researchers. Transbronchial biopsy results according to diffuse interstitial lung disease classification. Cryobiopsy versus forceps: MULTICRIO study. PLoS One. 2020;15:e0239114.

  32. Hetzel J, Maldonado F, Ravaglia C, Wells AU, Colby TV, Tomassetti S, Ryu JH, Fruchter O, Piciucchi S, Dubini A, Cavazza A, Chilosi M, Sverzellati N, Valeyre D, Leduc D, Walsh SLF, Gasparini S, Hetzel M, Hagmeyer L, Haentschel M, Eberhardt R, Darwiche K, Yarmus LB, Torrego A, Krishna G, Shah PL, Annema JT, Herth FJF, Poletti V. Transbronchial cryobiopsies for the diagnosis of diffuse parenchymal lung diseases: expert statement from the cryobiopsy working group on safety and utility and a call for standardization of the procedure. Respiration. 2018;95:188–200.

    PubMed  Google Scholar 

  33. Bronchoscopes - Pulmonology - Olympus Medical Systems. at https://www.olympus-europa.com/medical/en/Products-and-Solutions/Products/Pulmonology/Bronchoscopes.html.

  34. Wall CP, Gaensler EA, Carrington CB, Hayes JA. Comparison of transbronchial and open biopsies in chronic infiltrative lung diseases. Am Rev Respir Dis. 1981;123:280–5.

    CAS  PubMed  Google Scholar 

  35. Han Q, Luo Q, Xie J-X, Wu L-L, Liao L-Y, Zhang X-X, Chen R-C. Diagnostic yield and postoperative mortality associated with surgical lung biopsy for evaluation of interstitial lung diseases: a systematic review and meta-analysis. J Thorac Cardiovasc Surg. 2015;149:1394-401.e1.

    PubMed  Google Scholar 

  36. Tomassetti S, Piciucchi S, Tantalocco P, Dubini A, Poletti V. The multidisciplinary approach in the diagnosis of idiopathic pulmonary fibrosis: a patient case-based review. Eur Respir Rev. 2015;24:69–77.

    PubMed  Google Scholar 

  37. Romagnoli M, Colby TV, Berthet J-P, Gamez AS, Mallet J-P, Serre I, Cancellieri A, Cavazza A, Solovei L, Dell’Amore A, Dolci G, Guerrieri A, Reynaud P, Bommart S, Zompatori M, Dalpiaz G, Nava S, Trisolini R, Suehs CM, Vachier I, Molinari N, Bourdin A. Poor concordance between sequential transbronchial lung cryobiopsy and surgical lung biopsy in the diagnosis of diffuse interstitial lung diseases. Am J Respir Crit Care Med. 2019;199:1249–56.

    PubMed  Google Scholar 

  38. Raghu G, Lederer DJ, Rabe KF. Cryobiopsy for interstitial lung disease: the heat is on. Am J Respir Crit Care Med. 2019;199:1183–4.

    PubMed  PubMed Central  Google Scholar 

  39. Sharp C, McCabe M, Adamali H, Medford AR. Use of transbronchial cryobiopsy in the diagnosis of interstitial lung disease-a systematic review and cost analysis. QJM. 2017;110:207–14.

    CAS  PubMed  Google Scholar 

  40. Dhooria S, Agarwal R, Sehgal IS, Aggarwal AN, Goyal R, Guleria R, Singhal P, Shah SP, Gupta KB, Koolwal S, Akkaraju J, Annapoorni S, Bal A, Bansal A, Behera D, Chhajed PN, Dhamija A, Dhar R, Garg M, Gopal B, Hibare KR, James P, Jindal A, Jindal SK, Khan A, Kishore N, Koul PA, Kumar A, Kumar R, et al. Bronchoscopic lung cryobiopsy: an Indian association for bronchology position statement. Lung India. 2019;36:48–59.

    PubMed  PubMed Central  Google Scholar 

  41. Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. CA Cancer J Clin. 2015;65:87–108.

    PubMed  Google Scholar 

  42. Feinstein AR, Wells CK. A clinical-severity staging system for patients with lung cancer. Medicine. 1990;69:1–33.

    CAS  PubMed  Google Scholar 

  43. Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, Bray F. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2021;71:209–49.

    PubMed  Google Scholar 

  44. Oudkerk M, Liu S, Heuvelmans MA, Walter JE, Field JK. Lung cancer LDCT screening and mortality reduction - evidence, pitfalls and future perspectives. Nat Rev Clin Oncol. 2021;18:135–51.

    PubMed  Google Scholar 

  45. Liu X, Wang P, Zhang C, Ma Z. Epidermal growth factor receptor (EGFR): A rising star in the era of precision medicine of lung cancer. Oncotarget. 2017;8:50209–20.

    PubMed  PubMed Central  Google Scholar 

  46. Chen AC, Pastis NJ, Mahajan AK, Khandhar SJ, Simoff MJ, Machuzak MS, Cicenia J, Gildea TR, Silvestri GA. Robotic bronchoscopy for peripheral pulmonary lesions: a multicenter pilot and feasibility study (BENEFIT). Chest. 2021;159:845–52.

    PubMed  Google Scholar 

  47. Ost DE, Yeung S-CJ, Tanoue LT, Gould MK. Clinical and organizational factors in the initial evaluation of patients with lung cancer: diagnosis and management of lung cancer, 3rd ed: American College of Chest Physicians evidence-based clinical practice guidelines. Chest. 2013;143:e121S–41S.

  48. Schumann C, Hetzel M, Babiak AJ, Hetzel J, Merk T, Wibmer T, Lepper PM, Krüger S. Endobronchial tumor debulking with a flexible cryoprobe for immediate treatment of malignant stenosis. J Thorac Cardiovasc Surg. 2010;139:997–1000.

    PubMed  Google Scholar 

  49. Shepherd RW, Radchenko C. Bronchoscopic ablation techniques in the management of lung cancer. Ann Transl Med. 2019;7:362.

    CAS  PubMed  PubMed Central  Google Scholar 

  50. Saka H, Oki M, Kada A, Saito AM. An open-label, single-arm study of CRYO2 for debulking at the site of central airway obstruction or stenosis. In Vivo. 2019;33:1641–4.

    CAS  PubMed  PubMed Central  Google Scholar 

  51. Oberg C, Folch E, Santacruz JF. Management of malignant airway obstruction. AME Med J. 2018;3:115–115.

    Google Scholar 

  52. Chaddha U, Hogarth DK, Murgu S. Bronchoscopic ablative therapies for malignant central airway obstruction and peripheral lung tumors. Ann Am Thorac Soc. 2019;16:1220–9.

    PubMed  Google Scholar 

  53. Hetzel M, Hetzel J, Schumann C, Marx N, Babiak A. Cryorecanalization: a new approach for the immediate management of acute airway obstruction. J Thorac Cardiovasc Surg. 2004;127:1427–31.

    PubMed  Google Scholar 

  54. Rivera MP, Mehta AC, Wahidi MM. Establishing the diagnosis of lung cancer: diagnosis and management of lung cancer, 3rd ed: American College of Chest Physicians evidence-based clinical practice guidelines. Chest. 2013;143:e142S – e165.

    PubMed  Google Scholar 

  55. Schreiber G, McCrory DC. Performance characteristics of different modalities for diagnosis of suspected lung cancer: summary of published evidence. Chest. 2003;123:115S-128S.

    PubMed  Google Scholar 

  56. Hetzel J, Hetzel M, Hasel C, Moeller P, Babiak A. Old meets modern: the use of traditional cryoprobes in the age of molecular biology. Respiration. 2008;76:193–7.

    PubMed  Google Scholar 

  57. Kendall DM, Gal AA. Interpretation of tissue artifacts in transbronchial lung biopsy specimens. Ann Diagn Pathol. 2003;7:20–4.

    PubMed  Google Scholar 

  58. Simon M, Simon I, Tent PA, Todea DA, Haranguș A. Cryobiopsy in lung cancer diagnosis-a literature review. Medicina (Kaunas) 2021;57.

  59. Imabayashi T, Uchino J, Yoshimura A, Chihara Y, Tamiya N, Kaneko Y, Yamada T, Takayama K. Safety and usefulness of cryobiopsy and stamp cytology for the diagnosis of peripheral pulmonary lesions. Cancers (Basel). 2019;11.

  60. Haentschel M, Boeckeler M, Ehab A, Wagner R, Spengler W, Steger V, Boesmueller H, Horger M, Lewis RA, Fend F, Kanz L, Bonzheim I, Hetzel J. Cryobiopsy increases the EGFR detection rate in non-small cell lung cancer. Lung Cancer. 2020;141:56–63.

    PubMed  Google Scholar 

  61. Arimura K, Kondo M, Nagashima Y, Kanzaki M, Kobayashi F, Takeyama K, Tamaoki J, Tagaya E. Comparison of tumor cell numbers and 22C3 PD-L1 expression between cryobiopsy and transbronchial biopsy with endobronchial ultrasonography-guide sheath for lung cancer. Respir Res. 2019;20:185.

    PubMed  PubMed Central  Google Scholar 

  62. Nishida T, Matsumoto Y, Sasada S, Tanaka M, Nakai T, Fukai R, Ohe Y, Watanabe S-I, Motoi N. Feasibility study of cryobiopsy for practical pathological diagnosis of primary lung cancer including immunohistochemical assessment. Jpn J Clin Oncol. 2021;51:271–8.

    PubMed  Google Scholar 

  63. Hopkins PM, Aboyoun CL, Chhajed PN, Malouf MA, Plit ML, Rainer SP, Glanville AR. Prospective analysis of 1,235 transbronchial lung biopsies in lung transplant recipients. J Heart Lung Transplant. 2002;21:1062–7.

    PubMed  Google Scholar 

  64. Aboyoun CL, Tamm M, Chhajed PN, Hopkins P, Malouf MA, Rainer S, Glanville AR. Diagnostic value of follow-up transbronchial lung biopsy after lung rejection. Am J Respir Crit Care Med. 2001;164:460–3.

    CAS  PubMed  Google Scholar 

  65. Yarmus L, Akulian J, Gilbert C, Illei P, Shah P, Merlo C, Orens J, Feller-Kopman D. Cryoprobe transbronchial lung biopsy in patients after lung transplantation: a pilot safety study. Chest. 2013;143:621–6.

    PubMed  Google Scholar 

  66. Tomic R, Podgaetz E, Andrade RS, Dincer HE. Cryotechnology in diagnosing and treating lung diseases. J Bronchology Interv Pulmonol. 2015;22:76–84.

    PubMed  Google Scholar 

  67. Montero MA, de Gracia J, Culebras Amigo M, Mugnier J, Álvarez A, Berastegui C, Ortiz-Villalón C. The role of transbronchial cryobiopsy in lung transplantation. Histopathology. 2018;73:593–600.

    PubMed  Google Scholar 

  68. Loor K, Culebras M, Sansano I, Álvarez A, Berastegui C, de Gracia J. Optimization of transbronchial cryobiopsy in lung transplant recipients. Ann Thorac Surg. 2019;108:1052–8.

    PubMed  Google Scholar 

  69. Gershman E, Ridman E, Fridel L, Shtraichman O, Pertzov B, Rosengarten D, Rahman NA, Shitenberg D, Kramer MR. Efficacy and safety of trans-bronchial cryo in comparison with forceps biopsy in lung allograft recipients: analysis of 402 procedures. Clin Transplant. 2018;32:e13221.

  70. Almeida LM, Lima B, Mota PC, Melo N, Magalhães A, Pereira JM, Moura CS, Guimarães S, Morais A. Learning curve for transbronchial lung cryobiopsy in diffuse lung disease. Rev Port Pneumol. 2017. https://doi.org/10.1016/j.rppnen.2017.09.005.

    Article  PubMed  Google Scholar 

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  1. Division of Pulmonary, Allergy and Critical Care Medicine, University of Pittsburgh Medical Center, Pittsburgh, USA

    Jason Green, Sridesh Nath & Roy Semaan

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Green, J., Nath, S. & Semaan, R. Recent Advances in Transbronchial Lung Cryobiopsy. Curr Pulmonol Rep 11, 48–55 (2022). https://doi.org/10.1007/s13665-022-00288-4

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