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The effects of Baekho-tang on 1-Fluoro-2, 4-dinitrofluorobenzene-induced allergic contact dermatitis in BALB/c mice

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Abstract

Allergic contact dermatitis (ACD) is a cutaneous inflammatory response that is characterized skin lesion, increased cytokines secretion and splenomegaly. Baekho-tang (BHT) has been clinically used for the treatment of heat-related illnesses in Korea. Recently, it was reported that has an effect on epidemics such as hepatitis B, pneumonia, epidemic cerebrospinal meningitis and Japanese encephalitis. However, the effects of BHT on ACD is not yet fully studied. Therefore, the aims of this study was to determine the anti-allergic effects of BHT on 2, 4-dinitrofluorobenzene (DNFB)-induced ACD mice by investigating histopathologic examination of DNFB-induced inflamed tissues. Also, cytokines secretion in serum was determined using multiplex assay. The topical repeated application of DNFB on the dorsal skin of BALB/c mice were manifested the typical ACD. Treatment of different dose of BHT (20 or 80 mg/kg) attenuated DNFB-induced inflamed tissues such as erythema and crust. In addition, there is a trend for decreased serum cytokines levels in the treatment of high dose of BHT. Our results recommend that the high dose of BHT enhances the DNFB-induced ACD in mice.

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Acknowledgements

This study was supported by the Traditional Korean Medicine R&D program funded by the Ministry of Health & Welfare through the Korea Health Industry Development Institute (KHIDI) (HI14C0726).

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Authors and Affiliations

  1. Department of Science in Korean Medicine, Graduate School, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea

    In-Seung Lee, Kang-Hoon Kim, Jiyoung Park, Yumi Kim & Hyeung-Jin Jang

  2. College of Korean Medicine, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea

    In-Seung Lee, Hyun Jin Ahn, Kang-Hoon Kim, Jiyoung Park, Yumi Kim, Jin Woo Han, Dong Hyun Kim, Ji Hoon Jung, Hee Jae Jung & Hyeung-Jin Jang

  3. Department of Clinical Korean Medicine, Graduate School, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea

    Hyun Jin Ahn, Jin Woo Han, Dong Hyun Kim & Hee Jae Jung

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  1. In-Seung Lee
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  2. Hyun Jin Ahn
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  10. Hyeung-Jin Jang
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Correspondence to Hyeung-Jin Jang.

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The authors declare that there are no conflicts of interests.

Ethical Statement

The research was carried out according to the rules governing the use of laboratory animals as acceptable internationally and the experimental protocol was approved by the Animal Ethics Committee, Kyung Hee University, Seoul, Korea (Registration number: KHUASP(SE)-14–046).

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Lee, IS., Ahn, H.J., Kim, KH. et al. The effects of Baekho-tang on 1-Fluoro-2, 4-dinitrofluorobenzene-induced allergic contact dermatitis in BALB/c mice. Orient Pharm Exp Med 16, 349–354 (2016). https://doi.org/10.1007/s13596-016-0250-x

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