Abstract
The methanol extract of the bark of Entada rheedii has been fractionated to afford petroleum ether, dichloromethane, ethyl acetate and aqueous soluble fraction through modified Kupchan partitioning protocol and those fractions has been subjected to repeated-chromatographic separation by Sephadex size exclusion chromatography and purification processes to isolate the secondary metabolites. A total of seven compounds have been isolated from the bark of E. rheedii, which were identified as epicatechin (1), liquiritigenin (2), glabridin (3), 4′-O-methylglabridin (4), isoliquiritigenin (5), hispaglabridin A (6) and shinflavanone (7). This is the first report of isolation of these compounds from E. rheedii.
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Acknowledgments
The authors thank the Department of Pharmacy, King’s College London, United Kingdom for assisting with some NMR analysis of our samples. The research has been supported by University Grants Commission of Bangladesh (Grant No. Reg-Admin-3/2015/48711).
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The objectivity and transparency in research have been ensured and accepted principles of ethical policies of Oriental Pharmacy and Experimental Medicine have been followed. The experiment does not involve any human or animal subjects.
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Shafaat -Al- Mehedi, M., Hasan, C.M. & Haque, M.R. Isolation of flavonoids from the bark of Entada rheedii Spreng. Orient Pharm Exp Med 15, 347–351 (2015). https://doi.org/10.1007/s13596-015-0201-y
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DOI: https://doi.org/10.1007/s13596-015-0201-y