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Oral administration of betaine ameliorates ethanol-induced gastric injury in rats through its antioxidant effects

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Abstract

The aim of this study was to evaluate whether betaine ameliorates ethanol-induced gastric injury in rats. The morphological, histological and biochemical characteristics of gastric hemorrhagic lesions in ethanol-injured rats (n = 5/group) pretreated with betaine were analyzed and compared to non-treated controls. We compared the hemorrhagic dimensions using the Image J software, lipid peroxidation by malondialdehyde (MDA) concentration and inducible nitric oxide synthase (iNOS) immunoreactivity in the gastric mucosa among treatment groups. Oral administration of 250 mg/kg betaine significantly reduced gastric hemorrhage dimensions compared with the vehicle-treated control (p <0.05). Immunohistochemical analysis showed that the expression of iNOS and its byproduct nitrotyrosine were significantly reduced in betaine-pretreated rats compared to vehicle-treated rats with ethanol injury (p < 0.05). Lipid peroxidation was also significantly reduced in the ethanol-injured rats pretreated with betaine compared with the vehicle-treated ethanol-injured group (p <0.05). Collectively, these results suggest that pretreatment of betaine ameliorates ethanol-induced gastric mucosal injury, possibly through the inhibition of oxidative stress.

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Acknowledgement

This research was supported by the 2014 scientific promotion program funded by Jeju National University.

Conflict of Interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. School of Medicine, Jeju National University, Jeju, 690-756, Republic of Korea

    Meejung Ahn

  2. College of Veterinary Medicine, Jeju National University, Jeju, 690-756, Republic of Korea

    Yoonhyoung Kang & Jihwan Moon

  3. College of Veterinary Medicine, Kyungpook National University, Daegu, 702-701, Republic of Korea

    Seungjoon Kim

  4. Departments of Veterinary Anatomy, College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju, 500-757, Republic of Korea

    Changjong Moon

  5. Laboratory of Veterinary Anatomy College of Veterinary Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju, 690-756, Republic of Korea

    Taekyun Shin

Authors
  1. Meejung Ahn
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  2. Yoonhyoung Kang
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  3. Jihwan Moon
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  4. Seungjoon Kim
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  5. Changjong Moon
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Corresponding author

Correspondence to Taekyun Shin.

Additional information

Meejung Ahn and Yoonhyoung Kang equally contributed to this work.

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Ahn, M., Kang, Y., Moon, J. et al. Oral administration of betaine ameliorates ethanol-induced gastric injury in rats through its antioxidant effects. Orient Pharm Exp Med 14, 237–243 (2014). https://doi.org/10.1007/s13596-014-0158-2

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  • DOI: https://doi.org/10.1007/s13596-014-0158-2

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