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ACE inhibiton activity of standardized extract and fractions of Terminalia bellerica

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Abstract

The fruit of Terminalia bellerica L. (Combretaceae) is an important ingredient of ‘Triphala’, which is a popular Ayurvedic formulation traditionally used to treat hypertension, to reduce cardiac depression and to decreases the risk factors associated with the heart. This study aimed to investigate the angiotensin converting enzyme (ACE) inhibitory activity of T. bellerica. Standardized hydro alcoholic extract (TBHA) and its various subfractions including hexane fraction (TBH), ethyl acetate fraction (TBE), n-butanol fraction (TBB) and aqueous fraction (TBW) at the concentration of 10–1000 μg/ml together with standard Captopril 3.6 ng/ml was compared. TBE fraction was undertaken to isolate the gallic acid. Further quantification of gallic acid in the crude extract and fractions was made with HPLC. Among all fractions the activity was found to be maximum in TBE with an IC50 = 338.54 ± 18.34 μg/ml while crude TBHA and other fractions TBB, TBH and TBW were found less potent. Isolated gallic acid from the TBE fraction shown ACE inhibitory activity with IC50 of 257.29 ± 9.39 μg/ml. TBE found to contain maximum amount of gallic acid (71.05 ± 6.274 mg/g of extract). The presence of gallic acid along with other metabolites in the extract and fractions might be responsible for the ACE inhibitory activity. T. bellerica extract/fractions suggested its suitability as a functional food for pharmaceutical purpose to be used against hypertension and other related diseases.

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References

  • Anand KK, Singh B, Chandan BK, Gupta VN, Bhardwaj V (1997) 3,4,5-Trihydroxy benzoic acid (gallic acid), the hepatoprotective principle in the fruits of Terminalia belerica-bioassay guided activity. Pharmacol Res 36:315–21

    Article  PubMed  CAS  Google Scholar 

  • Anderson A, Anderson H (1997) Plants from Réunion Island with alleged antihypertensive and diuretic effects—an experimental and ethnobotanical evaluation. J Ethnopharmcol 58:189–206

    Article  Google Scholar 

  • Chanwitheesuk A, Teerawutgulrag A, Kilburn JD, Rakariyatham N (2007) Food Chem 100:1044–1048

    Article  CAS  Google Scholar 

  • Cheung HS, Cushman DW (1973) Inhibition of homogeneous angiotensin-converting enzyme of rabbit lung by synthetic venom peptides of Bothrops jararaca. Biochim Biophy Act 293:451–463

    Article  CAS  Google Scholar 

  • Chopra R, Nayar S, Chopra I (1956) Glossary of Indian medicinal plants. Council of Scientific and Industrial Research, New Delhi, pp 241–242

    Google Scholar 

  • Duncan AC, Jager AK, Johannes VS (1999) Screening of Zulu medicinal plants for angiotensin converting enzyme (ACE) inhibitors. J Ethnopharmcol 68:63–70

    Article  CAS  Google Scholar 

  • Dwivedi S, Pachori SB, Amrita (1994) Medicinal plants with hypotensive activity. Indian Pract 6:117–134

    Google Scholar 

  • Elizabeth KM (2005) Antimicrobial activity of Terminalia bellerica. Indian J Clin Biochem 20(2):150–153

    Article  PubMed  CAS  Google Scholar 

  • Hollenberg NK (1979) Pharmacologic interrupting the reninangiotensin system. Ann Rev Pharmacol 19:559–582

    Article  CAS  Google Scholar 

  • Liu JC, Hsu FL, Tsai JC, Chan P, Liu JYH, Thomas GN, Tomlison B, Lo MY, Lin JY (2003) Antihypertensive effects of tannins isolated from traditional Chinese herbs as non-specific inhibitors of angiotensin converting enzyme. Life Sci 73:1543–1555

    Article  PubMed  CAS  Google Scholar 

  • Mukherjee PK (2001) Evaluation of Indian traditional medicine. Drug Inf J 35:623–631

    Article  Google Scholar 

  • Mukherjee PK, Rai S, Bhattacharya S, Wahile A, Saha BP (2008) Marker analysis of polyherbal formulation, Triphala—A well known Indian traditional medicine. Indian J Trad Knowl 7:379–383

    Google Scholar 

  • Mukherji B (1953) In: Indian pharmaceutical codex. CSIR, New Delhi, India, 238

  • Nandy AK, Podder G, Sabu NP (1989) Triterpenoids and their glucosides from Terminalia bellerica. Phytochemistry 2:2769–2772

    Article  Google Scholar 

  • Ninahuaman MFML, Souccar C, Lapa AJ, Lima-Landman MTR (2007) ACE activity during the hypotension produced by standardized aqueous extract of Cecropia glaziovii Sneth: a comparative study to captopril effects in rats. Phytomedicine 14:321–327

    Article  PubMed  CAS  Google Scholar 

  • Nyman U, Joshi P, Madsen LB, Pederson TB, Pinstrup M, Rajasekharan S, George V, Pushpangadan P (1998) Ethnomedical information and in vitro screening for angiotensin-converting enzyme inhibition of plants utilized as traditional medicines in Gujarat, Rajasthan, and Kerala (India). J Ethnopharmcol 60:189–206

    Article  Google Scholar 

  • Park EJ, Jhon DY (2009) The antioxidant, angiotensin converting enzyme inhibition activity, and phenolic compounds of bamboo shoot extracts LWT. Food Sci and Tech 30:723–728

    Google Scholar 

  • Singh AK, Raghubanshi AS, Singh JS (2002) Medical ethnobotany of the tribals of Sonaghati of Sonbhadra district, Uttar Pradesh, India. J Ethnopharmacol 81:31–41

    Article  PubMed  CAS  Google Scholar 

  • Siddiqui HN (1963) Studies on Terminalia bellerica Roxb. Effect on bile secretion and pharmacodynamic properties. Indian J Pharmacol 25:297–302

    CAS  Google Scholar 

  • Somanadhan B, Varughese G, Palpu P, Sreedharan R, Gudiksen L, Smitt UW, Nyman U (1999) An ethnopharmacological survey for potential angiotensin converting enzyme inhibitors from India medicinal plants. J Ethnopharmacol 65:103–112

    Article  PubMed  CAS  Google Scholar 

  • Srivastava RD, Dwivedi S, Sreenivasan KK, Chandrashekhar CN (1992) Cardiovascular effects of Terminalia species of plants. Indian Drugs 29:144–149

    Google Scholar 

Download references

Acknowledgements

The authors wish to express their gratitude to the Council of Scientific and Industrial Research, Government of India for financial support through EMR project grant [F.No. - 60(0084)/08/EMR-II/2009]

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The authors declare that they have no conflict of interest with any matter.

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Authors and Affiliations

  1. School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, 700032, India

    Sushil Kumar Chaudhary, Pulok K. Mukherjee, Neelesh Kumar Nema, Santanu Bhadra & B. P. Saha

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  1. Sushil Kumar Chaudhary
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  2. Pulok K. Mukherjee
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  3. Neelesh Kumar Nema
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  4. Santanu Bhadra
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  5. B. P. Saha
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Correspondence to Pulok K. Mukherjee.

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Chaudhary, S.K., Mukherjee, P.K., Nema, N.K. et al. ACE inhibiton activity of standardized extract and fractions of Terminalia bellerica . Orient Pharm Exp Med 12, 273–277 (2012). https://doi.org/10.1007/s13596-012-0076-0

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  • DOI: https://doi.org/10.1007/s13596-012-0076-0

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