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Assessment of genetic diversity among okra genotypes using SSR markers

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Abstract

Okra (Abelmoschus esculentus) is an important nutritious vegetable. Despite its high economic and industrial value, very little attention has been paid to assess genetic diversity of okra at molecular level. For effective conservation and proper deployment of germplasm, a study on diversity analysis of okra germplasm was conducted with DNA markers. Microsatellite/Simple sequence repeat (SSR) markers were utilized to evaluate the genetic diversity among 96 accessions of Abelmoschus, of which 92 accessions were of A. esculentus and one accession each of A. tuberculatus, A. moschatus, A. moschatus subspecies tuberosus and A. manihot. A set of 40 SSR primers were tested, of which 30 primers gave reproducible amplification which were used further for diversity analysis. With a mean of 7.1 bands per SSR, DNA amplification with 30 SSRs generated a total 213 bands, of which 60.66 % were recorded polymorphic. Polymorphic information content ranged between 0.11 and 0.80 with an average of 0.52, indicating that the majority of primers were informative. The Jaccard’s coefficient ranged from 0.107 to 0.969. The UPGMA analysis grouped Abelmoschus genotypes into three main clusters at a cut-off of 0.20. Results of present study revealed that sufficient variation exists among the studied accessions and GAO-5 which was found highly diverse can be exploited for okra improvement. The outcome of present research would assist to make use of Ablemoschus germplasm for okra breeding.

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Abbreviations

EST:

Expressed sequence tag

SSR:

Simple sequence repeat

RAPD:

Random amplified polymorphic DNA

ISSR:

Inter simple sequence repeat

SRAP:

Sequence-related amplified polymorphism

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Author contribution

S.K. and M.J.P performed extraction of the DNA. C.B.P. and S.K.P. carried out polymerase chain reactions. M.K. and S.K. analysed the data. B.R.P. provided the seed material and manage the seedling growth. S.K. and R.S.F. contributed by designing the experiment, supervising the whole experiment and preparing the manuscript.

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Authors and Affiliations

  1. Department of Agricultural Biotechnology, Anand Agricultural University, Anand, 388 110, India

    Sushil Kumar, Mithil J. Parekh, Ranbir S. Fougat, Swati K. Patel, Chandni B. Patel, Mukesh Kumar & Babubhai R. Patel

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  1. Sushil Kumar
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  2. Mithil J. Parekh
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  3. Ranbir S. Fougat
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  4. Swati K. Patel
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  5. Chandni B. Patel
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  6. Mukesh Kumar
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  7. Babubhai R. Patel
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Correspondence to Sushil Kumar.

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Supplementary Fig. 1

SSR based dendrogram of the genetic similarities among twenty five accessions of Abelmoschus species achieved by the UPGMA method. The bootstrap values which are higher than 30 are shown. (PPTX 227 kb)

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Kumar, S., Parekh, M.J., Fougat, R.S. et al. Assessment of genetic diversity among okra genotypes using SSR markers. J. Plant Biochem. Biotechnol. 26, 172–178 (2017). https://doi.org/10.1007/s13562-016-0378-2

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  • DOI: https://doi.org/10.1007/s13562-016-0378-2

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