Abstract
The melon aphid, Aphis gossypii Glover (Hemiptera: Aphididae), is a highly polyphagous species. To investigate its genetic diversity among seasonal host plants, we conducted a field study in a diversified vegetable growing area in Beijing, China. The molecular marker mtDNA COI (1563-bp long) was used to analyze the genetic diversity of aphid populations collected from eight plant species belonging to families Malvaceae, Cucurbitaceae and Rosaceae. A total of 33 haplotypes were identified, five of which were shared haplotypes, while the remaining 28 were unique haplotypes. At least one haplotype was shared by all eight A. gossypii populations. Aphid populations showed high levels of nucleotide and haplotype diversity. The genetic diversity indices were maximal on the hibiscus (the primary host), whereas minimal on cucumber and strawberry (the secondary hosts). The analysis of molecular variance showed that most of the variance was distributed within populations. Based on the genetic distances, the eight populations can be divided into two groups, associated with primary and the secondary host plant, respectively. The aphids possibly migrated from Hibiscus to watermelon, thereafter dispersed from watermelon to other secondary host plants. Watermelon was an important host probably due to its early growing season and large planting area. Our results highlighted the need to target the population dispersal for effective control.
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References
Blackman R, Eastop V (2007) Taxonomic issues. In: Van Emden H, Harrington RV (eds) Aphids as crop pests. CAB International, London, pp 1–29
Brevault T, Carletto J, Linderme D, Vanlerberghe-Masutti F (2008) Genetic diversity of the cotton aphid Aphis gossypii in the unstable environment of a cotton growing area. Agric For Entomol 10:215–223. https://doi.org/10.1111/j.1461-9563.2008.00377.x
Carletto J, Lombaert E, Chavigny P, Brevault T, Lapchin L, Vanlerberghe-Masutti F (2009) Ecological specialization of the aphid Aphis gossypii Glover on cultivated host plants. Mol Ecol 18:2198–2212. https://doi.org/10.1111/j.1365-294X.2009.04190.x
Chen Y, Vanlerberghe-Masutti F, Wilson LJ, Barchia I, McLoon MO, Smith T, Herron GA (2013) Evidence of superclones in Australian cotton aphid Aphis gossypii Glover (Aphididae: Hemiptera). Pest Manag Sci 69:938–948. https://doi.org/10.1002/ps.3455
Clement M, Posada D, Crandall KA (2000) TCS: a computer program to estimate gene genealogies. Mol Ecol 9:1657–1659. https://doi.org/10.1046/j.1365-294x.2000.01020.x
De Barro P, Sherratt T, Brookes C, David O, Maclean N (1995) Spatial and temporal genetic variation in British field populations of the grain aphid Sitobion avenae (F.) (Hemiptera: Aphididae) studied using RAPD-PCR. P R Soc Lond B Bio 262:321–327. https://doi.org/10.1098/rspb.1995.0212
Fuller S, Chavigny P, Lapchin L, Vanlerberghe-Masutti F (1999) Variation in clonal diversity in glasshouse infestations of the aphid, Aphis gossypii Glover in southern France. Mol Ecol 8:1867–1877. https://doi.org/10.1046/j.1365-294x.1999.00782.x
Harrison RG (1989) Animal mitochondrial DNA as a genetic marker in population and evolutionary biology. Trends Ecol Evol 4:6–11. https://doi.org/10.1016/0169-5347(89)90006-2
Kennedy GG, Storer NP (2000) Life systems of polyphagous arthropod pests in temporally unstable cropping systems. Annu Rev Entomol 45:467–493. https://doi.org/10.1146/annurev.ento.45.1.467
Kim H, Hoelmer KA, Lee W, Kwon Y-D, Lee S (2010) Molecular and morphological identification of the soybean aphid and other Aphis species on the primary host Rhamnus davurica in Asia. Ann Entomol Soc Am 103:532–543. https://doi.org/10.1603/AN09166
Librado P, Rozas J (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451–1452. https://doi.org/10.1093/bioinformatics/btp187
Lokeshwari D, Kumar NK, Manjunatha H (2015) Molecular Diversity of the Aphis gossypii (Hemiptera: Aphididae): a potential vector of potyviruses (Potyviridae). Ann Entomol Soc Am 108:621–633. https://doi.org/10.1093/aesa/sav034
Lombaert E, Carletto J, Piotte C, Fauvergue X, Lecoq H, Vanlerberghe-Masutti F, Lapchin L (2009) Response of the melon aphid, Aphis gossypii, to host-plant resistance: evidence for high adaptive potential despite low genetic variability. Entomol Exp Appl 133:46–56. https://doi.org/10.1111/j.1570-7458.2009.00904.x
Luo J-Y et al (2016) The distribution and host shifts of cotton-melon aphids in Northern China. PLoS One. https://doi.org/10.1371/journal.pone.0152103
Lushai G, Loxdale HD (2004) Tracking movement in small insect pests, with special reference to aphid populations. Int J Pest Manage 50:307–315. https://doi.org/10.1080/09670870412331286049
Martinez-Torres D, Moya A, Hebert P, Simon J-C (1997) Geographic distribution and seasonal variation of mitochondrial DNA haplotypes in the aphid Rhopalosiphum padi (Hemiptera: Aphididae). Bull Entomol Res 87:161–167. https://doi.org/10.1017/S0007485300027309
Orantes L, Zhang W, Mian M, Michel A (2012) Maintaining genetic diversity and population panmixia through dispersal and not gene flow in a holocyclic heteroecious aphid species. Heredity 109:127–134. https://doi.org/10.1038/hdy.2012.21
Peccoud J, Ollivier A, Plantegenest M, Simon JC (2009) A continuum of genetic divergence from sympatric host races to species in the pea aphid complex. Proc Natl Acad Sci USA 106:7495–7500. https://doi.org/10.1073/pnas.0811117106
Simon C (1991) Molecular systematics at the species boundary: exploiting conserved and variable regions of the mitochondrial genome of animals via direct sequencing from amplified DNA. In: Molecular techniques in taxonomy. Springer, pp 33–71
Simon J-C, Rispe C, Sunnucks P (2002) Ecology and evolution of sex in aphids. Trends Ecol Evol 17:34–39. https://doi.org/10.1016/S0169-5347(01)02331-X
Thomas S, Boissot N, Vanlerberghe-Masutti F (2012a) What do spring migrants reveal about sex and host selection in the melon aphid? BMC Evol Biol 12:1. https://doi.org/10.1186/1471-2148-12-47
Thomas S, Dogimont C, Boissot N (2012b) Association between Aphis gossypii genotype and phenotype on melon accessions. Arthropod-Plant Inte 6:93–101. https://doi.org/10.1007/s11829-011-9155-2
Wang M, Xu L, Zhang R-J, Zhang G-M, Yu D-J (2014) Population genetic differentiation analysis of the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae) based on mtDNA COI gene. Acta Entomol Sin 57:1424–1438 (in Chinese with English summary)
Wang L et al (2016) Identification of Aphis gossypii Glover (Hemiptera: Aphididae) biotypes from different host plants in North China. PLoS One 11:1–15. https://doi.org/10.1371/journal.pone.0146345
Wang X-Y, Yang X-M, Lu B, Zhou L-H, Wu K-M (2017) Genetic variation and phylogeographic structure of the cotton aphid, Aphis gossypii, based on mitochondrial DNA and microsatellite markers. Sci Rep 7:1920. https://doi.org/10.1038/s41598-017-02105-4
Acknowledgements
We thank Accdon (www.accdon.com) for its linguistic assistance during the preparation of this manuscript. This study was supported by National Natural Science Foundation of China (No. 31501646), Foundation for the Talents by the Beijing municipal (2014000020124G080), and Scientific Research Project of Beijing Municipal Education Commission (KM201710020006).
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Dong, Z., Li, Y., Liu, S. et al. Genetic diversity of the melon aphid Aphis gossypii (Hemiptera: Aphididae) in a diversified vegetable growing area. Appl Entomol Zool 53, 259–266 (2018). https://doi.org/10.1007/s13355-018-0552-1
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DOI: https://doi.org/10.1007/s13355-018-0552-1