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Interaction between CA repeat microsatellites and HIF1α regulated the transcriptional activity of porcine IGF1 promoter

  • Animal Genetics • Original Paper
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Abstract

Insulin-like growth factor 1 (IGF1) is pivotal in the regulation of animal growth. Highly polymorphic CA repeat microsatellites have been identified in the IGF1 promoter region of different breeds of pigs. Previous studies showed that CA repeat microsatellites are associated with circulating IGF1 level. However, the mechanisms by which CA repeat microsatellites regulate IGF1 expression remain unclear. This study aimed to detect the association of CA repeat microsatellites with the transcriptional regulation of porcine IGF1 and the possible mechanisms. Results revealed that the number of CA repeats in porcine IGF1 promoter was 14–18, and a promoter with 14 or 15 CA repeats had a higher transcriptional activity (P < 0.01). Transcription factor hypoxia-inducible factor 1 subunit alpha (HIF1α) was confirmed to bind to the binding site upstream of CA repeat microsatellites. The microsatellites with 14 or 15 CA repeats were more sensitive to changes in the HIF1α expression level (P < 0.01). These results suggested that CA repeat microsatellites and HIF1α affected the transcriptional activity of each other in the regulation of IGF1 expression, thereby implying an interaction between them. Overall, this study provided novel evidence for elucidating the effects of CA repeat microsatellites on the transcriptional regulation of porcine IGF1.

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Funding

This work was financially supported by the National Natural Science Foundation of China (31672514) and the Technological Development Plan of Jilin Province (20170101024JC and 20190701070GH).

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Author notes

  1. Hongwei Geng and Linlin Hao contributed equally to this work as co-first authors.

Authors and Affiliations

  1. College of Animal Science, Jilin University, 5333 Xi’an Road, Changchun, 130062, Jilin, China

    Hongwei Geng, Linlin Hao, Yunyun Cheng, Chunli Wang, Shan Huang, Wenzhen Wei, Rui Yang, Haoyang Li, Songcai Liu & Hao Yu

  2. Five-Star Animal Health Pharmaceutical Factory of Jilin Province, Changchun, Jilin, 130062, China

    Songcai Liu

  3. The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun, Jilin, 130041, China

    Huayi Lu

Authors
  1. Hongwei Geng
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  2. Linlin Hao
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  3. Yunyun Cheng
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  4. Chunli Wang
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  5. Shan Huang
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  6. Wenzhen Wei
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  7. Rui Yang
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  8. Haoyang Li
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  9. Songcai Liu
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  10. Hao Yu
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  11. Huayi Lu
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Corresponding authors

Correspondence to Songcai Liu, Hao Yu or Huayi Lu.

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Communicated by: Maciej Szydlowski

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Geng, H., Hao, L., Cheng, Y. et al. Interaction between CA repeat microsatellites and HIF1α regulated the transcriptional activity of porcine IGF1 promoter. J Appl Genetics 61, 105–112 (2020). https://doi.org/10.1007/s13353-019-00529-4

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  • DOI: https://doi.org/10.1007/s13353-019-00529-4

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