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Molecular characterization of peste-des-petits ruminants virus from Nepal, 2005 to 2016

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Abstract

Outbreaks of peste-des-petits ruminants (PPR) has been reported regularly in Nepal since 1994. Despite this, there has been limited molecular characterization of circulating virus in the country. In this study a 351 bp segment of the nucleoprotein gene of the PPR virus (PPRV) was amplified and sequenced from ten samples collected between 2005 and 2016. Phylogenetic trees were estimated from these sequences using the maximum likelihood method confirming that all of the PPRV from the samples analysed belonged to the sub-clade IV of clade I of lineage IV and that they shared a common origin with other PPRV isolates in the region.

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Acknowledgements

This study was supported by funds from the IAEA Peaceful Uses Initiative (PUI) VETLAB Network.

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Authors and Affiliations

  1. Central Veterinary Laboratory, Kathmandu, Nepal

    Samjhana Kafle Pandey, Pragya Koirala & Manju Maharjan

  2. Animal Production and Health Laboratory Joint, FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, 1400, Vienna, Austria

    Charles Euloge Lamien, Giovanni Cattoli, William G. Dundon & Tirumala B. K. Settypalli

  3. APHL Joint FAO/IAEA Division, IAEA Laboratories, 2444, Seibersdorf, Austria

    William G. Dundon

Authors
  1. Samjhana Kafle Pandey
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  2. Pragya Koirala
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  3. Manju Maharjan
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  4. Charles Euloge Lamien
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  5. Giovanni Cattoli
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  6. William G. Dundon
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  7. Tirumala B. K. Settypalli
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Correspondence to William G. Dundon.

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Pandey, S.K., Koirala, P., Maharjan, M. et al. Molecular characterization of peste-des-petits ruminants virus from Nepal, 2005 to 2016. VirusDis. 30, 315–318 (2019). https://doi.org/10.1007/s13337-018-0504-y

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  • DOI: https://doi.org/10.1007/s13337-018-0504-y

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