Abstract
Herein, miRNA candidates relevant to mycosis fungoides were investigated to provide data on the molecular mechanisms underlying the pathogenesis of the disease. The miRNA expression profile of skin biopsies from patients with tumor stage MF (tMF) and normal donors was compared using miRNA microarrays. Overall, 154 miRNAs were found differentially expressed between tMF and the control cohort with the majority of them being up-regulated (57 %). Among the upregulated miRNAs, miR-3177, miR-514b-3p, miR-1267, and miR-1282 were exclusively detected in 70 % of tMF. Additional upregulated miRNAs included miR-34a, miR-29a, let-7a*, and miR-210, while miR-200c* was identified among the downregulated ones. Quantitative real-time polymerase chain reaction was used to further investigate the expression profiles of miR-34a and miR-29a and validated the overexpression of miR-34a. Enrichment studies revealed that the target genes of the differentially expressed miRNAs were important in several cancer-related signaling pathways. The overlapping relationship of the target genes among tMF, Sezary syndrome, and atopic dermatitis revealed several common and disease-specific genes. Collectively, our study modulated miR-34a as a candidate oncogenic molecule and miR-29a as a putative tumor suppressor highlighting their promising potential in the molecular pathogenesis of tMF.
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EP conceived and designed the study, performed patients’ diagnosis and treatment, and drafted the manuscript. MBr performed all experiments, participated in the interpretation of data analyses, and drafted the manuscript. LA and TG assisted in all experiments’ performance. MB, EA, and GS performed bioinformatics analyses. VN and CA performed tumor diagnosis and treated patients. SKT, DR, and AK participated in the coordination of the study. AK also participated in study design and supervised the study. All authors approved the final manuscript.
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E Papadavid and M Braoudaki contributed equally to this work.
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Papadavid, E., Braoudaki, M., Bourdakou, M. et al. Aberrant microRNA expression in tumor mycosis fungoides. Tumor Biol. 37, 14667–14675 (2016). https://doi.org/10.1007/s13277-016-5325-2
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DOI: https://doi.org/10.1007/s13277-016-5325-2